A RAPID PROCEDURE FOR THE ISOLATION OF RNA FROM HIGH-PHENOLIC-CONTAINING TISSUES OF PECAN

被引：41

作者：

LEVI, A ^{[1
]}

GALAU, GA ^{[1
]}

WETZSTEIN, HY ^{[1
]}

机构：

[1] UNIV GEORGIA,DEPT BOT,ATHENS,GA 30602

来源：

HORTSCIENCE | 1992年 / 27卷 / 12期

关键词：

CARYA-ILLINOINENSIS; NUCLEIC ACID; EMBRYOS;

D O I：

10.21273/HORTSCI.27.12.1316

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

A simple and efficient protocol is reported for the isolation of RNA from embryos and leaves of pecan [Carya illinoinensis (Wangenh.) K. Koch]. The method relies on suppression of the polyphenols from interaction with the RNA and their rapid removal from the homogenate by chloroform extraction. This method produced abundant amounts of high-quality RNA. This protocol is likely to be useful for Juglandaceous species and other recalcitrant plants with high levels of phenolic compounds.

引用

页码：1316 / 1318

页数：3

共 14 条

[1]

BAKER SS, 1990, BIOTECHNIQUES, V9, P268

[2] ABSCISIC-ACID INDUCTION OF CLONED COTTON LATE EMBRYOGENESIS-ABUNDANT (LEA) MESSENGER-RNAS [J].