THE DROSOPHILA TISSUE-SPECIFIC FACTOR GRAINYHEAD CONTAINS NOVEL DNA-BINDING AND DIMERIZATION DOMAINS WHICH ARE CONSERVED IN THE HUMAN PROTEIN CP2

被引：68

作者：

UV, AE ^{[1
]}

THOMPSON, CRL ^{[1
]}

BRAY, SJ ^{[1
]}

机构：

[1] UNIV CAMBRIDGE,DEPT ANAT,CAMBRIDGE CB2 3DY,ENGLAND

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1994年 / 14卷 / 06期

关键词：

D O I：

10.1128/MCB.14.6.4020

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have mapped the regions in the Drosophila melanogaster tissue-specific transcription factor Grainyhead that are required for DNA binding and dimerization. These functional domains correspond to regions conserved between Grainyhead and the vertebrate transcription factor CP2, which we show has similar activities. The identified DNA-binding domain is large (263 amino acids) but contains a smaller core that is able to interact with DNA at approximately 400-fold lower affinity. The major dimerization domain is located in a separate region of the protein and is required to stabilize the interactions with DNA. Our data also suggest that Grainyhead activity can be modulated by an N-terminal inhibitory domain.

引用

页码：4020 / 4031

页数：12

共 52 条

[1] CELL-TYPE-SPECIFIC MECHANISMS OF TRANSCRIPTIONAL REPRESSION BY THE HOMEOTIC GENE-PRODUCTS UBX AND ABD-A IN DROSOPHILA EMBRYOS [J].