CRYSTALLIZATION AND PRELIMINARY DIFFRACTION STUDIES OF ESCHERICHIA-COLI LYSYL-TRANSFER-RNA SYNTHETASE (LYSU)

被引：13

作者：

ONESTI, S

THEOCLITOU, ME

WITTUNG, L

MILLER, AD

PLATEAU, P

BLANQUET, S

BRICK, P

PERNILLA, E

机构：

[1] UNIV LONDON IMPERIAL COLL SCI & TECHNOL,DEPT CHEM,LONDON SW7 2BZ,ENGLAND

[2] ECOLE POLYTECH,CNRS,BIOCHEM LAB,UNITE RECH 240,F-91128 PALAISEAU,FRANCE

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1994年 / 243卷 / 01期

关键词：

CLASS II AMINOACYL-TRANSFER-RNA SYNTHETASES; LYSU GENE PRODUCT; HEAT-SHOCK; APPPPA; X-RAY CRYSTALLOGRAPHY;

D O I：

10.1006/jmbi.1994.1635

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Crystals of Escherichia coli lysyl tRNA synthetase (lysU gene product) have been obtained by vapour diffusion techniques. Three different crystal forms could be grown under similar conditions. The crystals that have been chosen for the structure determination belong to space group C222(1) with cell dimensions a = 144.3 Angstrom, b = 257.8 Angstrom, c = 182.1 Angstrom and contain three monomers in the asymmetric unit. They diffract to at least 2.1 Angstrom resolution, but are very sensitive to radiation damage.

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页码：123 / 125

页数：3

相关论文

共 19 条

[1] ALTERATION OF ADENYL DINUCLEOTIDE METABOLISM BY ENVIRONMENTAL-STRESS [J].

BAKER, JC ;

JACOBSON, MK .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (08) :2350-2352

[2] INVIVO SYNTHESIS OF ADENYLYLATED BIS(5'-NUCLEOSIDYL) TETRAPHOSPHATES (AP4N) BY ESCHERICHIA-COLI AMINOACYL-TRANSFER RNA-SYNTHETASES [J].

BREVET, A ;

CHEN, J ;

LEVEQUE, F ;

PLATEAU, P ;

BLANQUET, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (21) :8275-8279

[3]

BRICK P, 1988, J MOL BIOL, V208, P83

[4] CRYSTALLOGRAPHIC STUDY AT 2.5A RESOLUTION OF THE INTERACTION OF METHIONYL-TRANSFER-RNA SYNTHETASE FROM ESCHERICHIA-COLI WITH ATP [J].

BRUNIE, S ;

ZELWER, C ;

RISLER, JL .

JOURNAL OF MOLECULAR BIOLOGY, 1990, 216 (02) :411-424

[5] LYSYL-TRANSFER RNA-SYNTHETASE FROM ESCHERICHIA-COLI-K12 - CHROMATOGRAPHIC HETEROGENEITY AND THE LYSU-GENE PRODUCT [J].

CHARLIER, J ;

SANCHEZ, R .

BIOCHEMICAL JOURNAL, 1987, 248 (01) :43-51

[6] ROLES OF THE 2 LYSYL-TRANSFER-RNA SYNTHETASES OF ESCHERICHIA-COLI - ANALYSIS OF NUCLEOTIDE-SEQUENCES AND MUTANT BEHAVIOR [J].

CLARK, RL ;

NEIDHARDT, FC .

JOURNAL OF BACTERIOLOGY, 1990, 172 (06) :3237-3243

[7] A 2ND CLASS OF SYNTHETASE STRUCTURE REVEALED BY X-RAY-ANALYSIS OF ESCHERICHIA-COLI SERYL-TRANSFER RNA-SYNTHETASE AT 2.5-A [J].

CUSACK, S ;

BERTHETCOLOMINAS, C ;

HARTLEIN, M ;

NASSAR, N ;

LEBERMAN, R .

NATURE, 1990, 347 (6290) :249-255

[8] PARTITION OF TRANSFER-RNA SYNTHETASES INTO 2 CLASSES BASED ON MUTUALLY EXCLUSIVE SETS OF SEQUENCE MOTIFS [J].

ERIANI, G ;

DELARUE, M ;

POCH, O ;

GANGLOFF, J ;

MORAS, D .

NATURE, 1990, 347 (6289) :203-206

[9] ESCHERICHIA-COLI LEUCINE-RESPONSIVE REGULATORY PROTEIN (LRP) CONTROLS LYSYL-TRANSFER RNA-SYNTHETASE EXPRESSION [J].

GAZEAU, M ;

DELORT, F ;

DESSEN, P ;

BLANQUET, S ;

PLATEAU, P .

FEBS LETTERS, 1992, 300 (03) :254-258

[10] APPPPA BINDS TO SEVERAL PROTEINS IN ESCHERICHIA-COLI, INCLUDING THE HEAT-SHOCK AND OXIDATIVE STRESS PROTEINS DNAK, GROEL, E89, C45 AND C40 [J].

JOHNSTONE, DB ;

FARR, SB .

EMBO JOURNAL, 1991, 10 (12) :3897-3904