IMMUNOHISTOLOGICAL IDENTIFICATION OF INTERLEUKIN-1 ACTIVATED CHONDROCYTES

被引：13

作者：

DINGLE, JT ^{[1
]}

DAVIES, ME ^{[1
]}

MATIVI, BY ^{[1
]}

MIDDLETON, HF ^{[1
]}

机构：

[1] STRANGEWAYS RES LAB,DEPT TISSUE PHYSIOL,WORTS CAUSEWAY,CAMBRIDGE CB1 4RN,ENGLAND

来源：

ANNALS OF THE RHEUMATIC DISEASES | 1990年 / 49卷 / 11期

关键词：

D O I：

10.1136/ard.49.11.889

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Interleukin-I activated chondrocytes have been identified in pig articular cartilage by inmmunolocalisation using a polyclonal antiserum recognising cytokine induced surface epitopes. Although chondrocytes with membrane staining were noted in all zones of the cartilage, only a proportion of the cells were positive within the range of antiserum dilution used (1:100-1:2000). Maximum staining was found after fours days' treatment of cultured cartilage with pig IL-lα, though weak staining was just detectable after treatment for one day at high antiserum concentration. Immunoreactivity was also shown in cartilage which had been cultured with human recombinant IL-lα.

引用

页码：889 / 892

页数：4

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