A STANDARDIZED EXPERIMENTAL FRACTURE IN THE MOUSE TIBIA

被引:174
作者
HILTUNEN, A
VUORIO, E
ARO, HT
机构
[1] UNIV TURKU, DEPT SURG, SF-20520 TURKU, FINLAND
[2] UNIV TURKU, DEPT MED BIOCHEM, SF-20520 TURKU, FINLAND
关键词
D O I
10.1002/jor.1100110219
中图分类号
R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学(修复外科学)];
学科分类号
摘要
The increased use of transgenic mice as experimental animals provides new opportunities to study the biology of fracture repair. We have developed a technique for the production of a standard closed experimental fracture in the mouse tibia. A 0.2 mm stainless-steel rod was introduced into the medullary cavity and the pre-nailed tibial shaft was fractured by an impact device, which resulted in a reproducible transverse or slightly oblique fracture pattern. The intramedullary rod maintained axial alignment, and the fractures united without displacement. On the basis of measurements of callus geometry, four-point bending tests, biochemical analyses, and quantitative histology, the progress of callus formation and remodeling occurred in a predictable sequence of healing phases. The ultimate bending loads of the fractures increased with time, reaching 74% of the strength of intact control tibias in 4 weeks. The stiffness values of the fractures returned to normal levels and, as determined radiographically, the fractures united by external callus in 4 weeks. Radiographically, callus size, cross-sectional callus area, and callus mass peaked at 2 weeks and decreased thereafter, indicating the start of external remodeling. Histologically, the amount of mesenchymal tissue was maximal at days 5 and 7. The callus cartilage area peaked at day 9; at its maximum, it accounted for 46% of the total callus area. Early periosteal formation of membranous new bone, followed by endochondral ossification, resulted in a linear increase of callus bone during the healing process. The healing sequence of the mouse tibial fracture was similar to that seen in the rat tibia. The major difference is the small size of the mouse, which makes the surgical technique and anesthetic procedures more demanding.
引用
收藏
页码:305 / 312
页数:8
相关论文
共 23 条
[1]   DETERMINATION OF CALLUS QUANTITY IN 4-WEEK-OLD FRACTURES OF THE RAT TIBIA [J].
ARO, H ;
EEROLA, E ;
AHO, AJ .
JOURNAL OF ORTHOPAEDIC RESEARCH, 1985, 3 (01) :101-108
[2]   PREDICTION OF PROPERTIES OF FRACTURE CALLUS BY MEASUREMENT OF MINERAL DENSITY USING MICRO-BONE DENSITOMETRY [J].
ARO, HT ;
WIPPERMANN, BW ;
HODGSON, SF ;
WAHNER, HW ;
LEWALLEN, DG ;
CHAO, EYS .
JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME, 1989, 71A (07) :1020-1030
[3]  
BONNARENS F, 1984, Journal of Orthopaedic Research, V2, P97, DOI 10.1002/jor.1100020115
[4]   STANDARD TEST FOR LABORATORY ANIMAL BONE [J].
BURSTEIN, AH ;
FRANKEL, VH .
JOURNAL OF BIOMECHANICS, 1971, 4 (02) :155-&
[5]   REDUCED AMOUNTS OF CARTILAGE COLLAGEN FIBRILS AND GROWTH PLATE ANOMALIES IN TRANSGENIC MICE HARBORING A GLYCINE-TO-CYSTEINE MUTATION IN THE MOUSE TYPE-II PROCOLLAGEN ALPHA-1-CHAIN GENE [J].
GAROFALO, S ;
VUORIO, E ;
METSARANTA, M ;
ROSATI, R ;
TOMAN, D ;
VAUGHAN, J ;
LOZANO, G ;
MAYNE, R ;
ELLARD, J ;
HORTON, W ;
DECROMBRUGGHE, B .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (21) :9648-9652
[6]  
GREIFF J, 1978, INJURY, V9, P278
[7]  
GUDE WD, 1982, HISTOLOGICAL ATLAS L, P29
[8]   STUDIES ON MECHANICAL PROPERTIES OF HEALING EXPERIMENTAL FRACTURES [J].
HENRY, AN ;
FREEMAN, MAR ;
SWANSON, SAV .
PROCEEDINGS OF THE ROYAL SOCIETY OF MEDICINE-LONDON, 1968, 61 (09) :902-&
[9]  
HOGAN B, 1986, MANIPULATING MOUSE E, P271
[10]   ACIDIC FIBROBLAST GROWTH-FACTOR (AFGF) INJECTION STIMULATES CARTILAGE ENLARGEMENT AND INHIBITS CARTILAGE GENE-EXPRESSION IN RAT FRACTURE-HEALING [J].
JINGUSHI, S ;
HEYDEMANN, A ;
KANA, SK ;
MACEY, LR ;
BOLANDER, ME .
JOURNAL OF ORTHOPAEDIC RESEARCH, 1990, 8 (03) :364-371