REDUCTION OF MONO(ADP-RIBOSYL)ATION OF HISTONES IN RAT TESTIS BY GONADOTROPIN-TESTOSTERONE SYSTEM

被引：7

作者：

KUROKAWA, T

FUJIMURA, Y

TAKAHASHI, K

CHONO, E

ISHIBASHI, S

机构：

[1] Department Physiological Chemistry, Hiroshima University School Medicine, Hiroshima, Minami-ku 734, Kasumi

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 215卷 / 03期

关键词：

D O I：

10.1006/bbrc.1995.2535

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mono(ADP-ribose) synthetase activity in the nuclear fraction of immature rat testis was investigated in reference to the effect of testosterone. When the nuclear fraction was incubated with [P-32] NAD in the presence of nicotinamide, an inhibitor of poly (ADP-ribose) synthetase, some proteins with a molecular mass of 14.4 similar to 21.5 kDa were ADP-ribosylated. In these proteins, H2B and H3 histones were found by sequencing 20 amino acids in the N-terminus. Their incorporations of ADP-ribose moiety were significantly decreased in immature rats at 4 hr after the subcutaneous injection of testosterone. Furthermore, the treatments with LH and FSH of the immature rats reduced mono(ADP-ribosyl)ations of H2B and H3 histones in a time-dependent manner. These results suggest that the testicular mono(ADP-ribose) synthetase activity is under the control of gonadotropin-testosterone system and is possibly related to differentiation of the testis. (C) 1995 Academic Press, Inc.

引用

页码：808 / 813

页数：6

共 22 条

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