HOMEOBOX GENE-EXPRESSION IN HUMAN OOCYTES AND PREEMBRYOS

被引:21
作者
VERLINSKY, Y
MOROZOV, G
GINDILIS, V
STROM, CM
FREIDIN, M
RECHITSKY, S
VERLINSKY, O
IVAKHNENKO, V
ZDANOVSKY, V
KULIEV, A
机构
[1] Reproductive Genetics Institute, Illinois Masonic Medical Center, Chicago, Illinois
关键词
HOMEOBOX; DEVELOPMENT; PREEMBRYOS; INTRON SPANNING PRIMERS;
D O I
10.1002/mrd.1080410202
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Homeobox gene expression in human preimplantation development has not been established. We used reverse transcriptase-polymerase chain reaction (RT-PCR) with intron spanning primer sets to investigate the presence of mRNA of homeobox genes in human oocytes and preembryos. RT-PCR products obtained from normal and unfertilized oocytes, from cleaving normal and triploid embryos, and from morulae and blastocysts were cloned, sequenced, and analyzed for the presence of homeobox sequences. The presence of mRNA of homeoboxes HoxA4 and HoxA7 was demonstrated; HoxA4 was present in normal and unfertilized oocytes and also in a 4-cell embryo. HoxA7 was present in normal oocytes and cleaving triploid embryos. (C) 1995 Wiley-Liss, Inc.
引用
收藏
页码:127 / 132
页数:6
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