HUMAN ADENOVIRUS SEROTYPE-3 (AD3) AND THE AD3 FIBER PROTEIN BIND TO A 130-KDA MEMBRANE-PROTEIN ON HELA-CELLS

被引：20

作者：

DIGUILMI, AM ^{[1
]}

BARGE, A ^{[1
]}

KITTS, P ^{[1
]}

GOUT, E ^{[1
]}

CHROBOCZEK, J ^{[1
]}

机构：

[1] INST BIOL STRUCT,F-38027 GRENOBLE,FRANCE

来源：

VIRUS RESEARCH | 1995年 / 38卷 / 01期

关键词：

ADENOVIRUS; FIBER PROTEIN; BACULOVIRUS EXPRESSION SYSTEM; CELLULAR RECEPTOR; MEMBRANE PROTEIN;

D O I：

10.1016/0168-1702(95)00043-P

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The fiber protein of adenovirus mediates the interaction of adenovirus with cell membrane receptors. We have produced the Ad3 fiber protein in the baculovirus expression system. Biochemical, morphological and functional analyses showed that the recombinant fiber was properly folded and functionally competent. The specific binding of Ad3 virus to two HeLa membrane proteins of 130 and 100 kDa was demonstrated with an overlay protein binding assay. In the same assay, Ad3 fiber only recognized the 130-kDa protein. Divalent cations seemed to be important for the interaction of both virus and fiber with these proteins.

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页码：71 / 81

页数：11

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