PURIFICATION AND CHARACTERIZATION OF 2 XYLANASES FROM TRICHODERMA-LONGIBRACHIATUM

被引:41
作者
ROYER, JC [1 ]
NAKAS, JP [1 ]
机构
[1] SUNY COLL ENVIRONM SCI & FORESTRY, SYRACUSE, NY 13210 USA
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1991年 / 202卷 / 02期
关键词
D O I
10.1111/j.1432-1033.1991.tb16404.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Two endoxylanases were purified from the culture medium of Trichoderma longibrachiatum. Both enzymes were highly basic, and lacked activity on carboxymethyl-cellulose. An enzyme of 21.5 kDa (xylanase A) had a specific activity of 510 U/mg protein, a K(m) of 0.15 mg soluble xylan/ml, possessed transglycosidase activity and generated xylobiose and xylotriose as the major endproducts from xylan or xylose oligomers. A larger enzyme of 33 kDa (xylanase B) had a specific activity of 131 U/mg protein, a K(m) of 0.19 mg soluble xylan/ml, lacked detectable transglycosidase activity and generated xylobiose and xylose as major endproducts from xylan and xylose oligomers. Xylotriose was the smallest oligomer attacked by both enzymes. In addition, xylotriose inhibited hydrolysis of xylopentanose by both enzymes, while xylobiose appeared to inhibit xylanase B, but not xylanase A.
引用
收藏
页码:521 / 529
页数:9
相关论文
共 41 条
[11]  
FOURNIER R, 1985, BIOTECHNOL BIOENG, V27, P539
[12]   PURIFICATION AND CHARACTERIZATION OF ENDO-XYLANASES FROM ASPERGILLUS-NIGER .1. 2 ISOZYMES ACTIVE ON XYLAN BACKBONES NEAR BRANCH-POINTS [J].
FREDERICK, MM ;
KIANG, CH ;
FREDERICK, JR ;
REILLY, PJ .
BIOTECHNOLOGY AND BIOENGINEERING, 1985, 27 (04) :524-532
[13]   PURIFICATION AND CHARACTERIZATION OF A XYLOBIOSE-PRODUCING AND XYLOSE-PRODUCING ENDO-XYLANASE FROM ASPERGILLUS-NIGER [J].
FREDERICK, MM ;
FREDERICK, JR ;
FRATZKE, AR ;
REILLY, PJ .
CARBOHYDRATE RESEARCH, 1981, 97 (01) :87-103
[14]   ANALYTICAL ISOELECTRIC-FOCUSING USING A HIGH-VOLTAGE VERTICAL SLAB POLYACRYLAMIDE-GEL SYSTEM [J].
GIULIAN, GG ;
MOSS, RL ;
GREASER, M .
ANALYTICAL BIOCHEMISTRY, 1984, 142 (02) :421-436
[15]   STUDIES ON XYLAN DEGRADING ENZYMES .1. PURIFICATION AND CHARACTERIZATION OF ENDO-1,4-BETA-XYLANASE FROM ASPERGILLUS-NIGER STR-14 [J].
GORBACHEVA, IV ;
RODIONOVA, NA .
BIOCHIMICA ET BIOPHYSICA ACTA, 1977, 484 (01) :79-93
[16]   SPECIFICITY OF CELLULASE AND BETA-XYLANASE INDUCTION IN TRICHODERMA-REESEI QM-9414 [J].
HRMOVA, M ;
BIELY, P ;
VRSANSKA, M .
ARCHIVES OF MICROBIOLOGY, 1986, 144 (03) :307-311
[17]  
Jeffries T W, 1983, Adv Biochem Eng Biotechnol, V27, P1
[18]   XYLANASE ACTIVITY OF AN ENDO-CELLULASE OF CARBOXYMETHYL-CELLULASE TYPE FROM IRPEX-LACTEUS (POLYPORUS-TULIPIFERAE) [J].
KANDA, T ;
WAKABAYASHI, K ;
NISIZAWA, K .
JOURNAL OF BIOCHEMISTRY, 1976, 79 (05) :989-995
[19]  
Kaufman S, 1971, METHOD ENZYMOL, V22, P233
[20]   ASSAY OF XYLANASE AND XYLOSIDASE ACTIVITIES IN BACTERIAL AND FUNGAL CULTURES [J].
KHAN, AW ;
TREMBLAY, D ;
LEDUY, A .
ENZYME AND MICROBIAL TECHNOLOGY, 1986, 8 (06) :373-377