ASSOCIATION OF THE HUMAN INVARIANT CHAIN WITH H-2-DB CLASS-I MOLECULES

被引:31
作者
CERUNDOLO, V
ELLIOTT, T
ELVIN, J
BASTIN, J
TOWNSEND, A
机构
[1] Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, Headington
关键词
D O I
10.1002/eji.1830220910
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
We describe two proteins of 24 kDa and 33 kDa (p24 and p33) which associate with H-20 K(b) and H-2 D(b) molecules. respectively, in human cells transfected with H-2 K(b) and H-2 D(b) genes. This association is particularly clear in the mutant cell line T2, in which association of endogenous peptide with newly synthesized class I molecules may not occur (V. Cerundolo et al., Nature 1990. 345: 449). We show that p33 is the 33-kDa form of the human invariant chain which is resident in the endoplasmic reticulum of T2 cells (P. Cresswell. Cold Spring Harbor Symp. Quant. Biol. 1989. LIV: 309). The stability of the invariant chain H-2 D(b) complex is critically dependent upon occupation of the class I binding site by peptide ligand. In the absence of peptide, the complex is stable at 4-degrees-C whereas following exposure to peptide, the invariant chain dissociates rapidly from H-2 D(b) molecules (half-life of 30 min at 4-degrees-C). Although the interaction between the human invariant chain and murine H-2 D(b) is unlikely to have any functional significance, the peptide-induced dissociation of the invariant chain is consistent with a conformational change in H-2 D(b) on peptide binding.
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页码:2243 / 2248
页数:6
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