D-GLUCOSE XYLOSE ISOMERASE FROM STREPTOMYCES - DIFFERENTIAL ROLES OF MAGNESIUM AND COBALT IONS

被引：17

作者：

GAIKWAD, SM ^{[1
]}

RAO, MB ^{[1
]}

DESHPANDE, VV ^{[1
]}

机构：

[1] NATL CHEM LAB,DIV BIOCHEM SCI,POONA 411008,MAHARASHTRA,INDIA

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1992年 / 14卷 / 04期

关键词：

GLUCOSE XYLOSE ISOMERASE; METAL ION EFFECT; SUBUNIT DISSOCIATION;

D O I：

10.1016/0141-0229(92)90158-K

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The presence of Mg2+ and Co2+ is essential for the maximum activity of glucose/xylose isomerase (GXI) from Streptomyces sp. NCIM 2730. Mg2+ is superior to Co2+ as an activator but fails to protect the enzyme against inactivation by denaturants. The inactivation of GXI by urea, guanidinium chloride, heat, and acid pH is protected by Co2+. Thermal dissociation of the active dimer into inactive monomer was partly prevented by Co2+, suggesting that Co2+ has an essential role in holding the ordered conformation, especially the quaternary structure of the enzyme.

引用

页码：317 / 320

页数：4

共 12 条

[1] D-XYLOSE ISOMERASE FROM STREPTOMYCES-VIOLACEUS-RUBER - OPTIMIZATION OF FERMENTATION CONDITIONS [J].

CALLENS, M ;

KERSTERSHILDERSON, H ;

VANOPSTAL, O ;

DEBRUYNE, CK .

BIOTECHNOLOGY LETTERS, 1985, 7 (08) :597-600

[2]

CHEN WP, 1980, PROCESS BIOCHEM, V15, P36

[3] MECHANISM FOR ALDOSE-KETOSE INTERCONVERSION BY D-XYLOSE ISOMERASE INVOLVING RING-OPENING FOLLOWED BY A 1,2-HYDRIDE SHIFT [J].

COLLYER, CA ;

HENRICK, K ;

BLOW, DM .

JOURNAL OF MOLECULAR BIOLOGY, 1990, 212 (01) :211-235

[4] STUDIES ON D-GLUCOSE-ISOMERIZING ENZYME FROM BACILLUS-COAGULANS, STRAIN HN-68 .6. ROLE OF METAL IONS ON ISOMERIZATION OF D-GLUCOSE AND D-XYLOSE BY ENZYME [J].

DANNO, G .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1971, 35 (07) :997-&

[5] STREPTOMYCES GLUCOSE XYLOSE ISOMERASE HAS A SINGLE ACTIVE-SITE FOR GLUCOSE AND XYLOSE [J].

GAIKWAD, SM ;

PAWAR, HS ;

VARTAK, HG ;

DESHPANDE, VV .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 159 (02) :457-463

[6] EVIDENCE FOR THE ESSENTIAL HISTIDINE RESIDUE AT THE ACTIVE-SITE OF GLUCOSE XYLOSE ISOMERASE FROM STREPTOMYCES [J].

GAIKWAD, SM ;

MORE, MW ;

VARTAK, HG ;

DESHPANDE, VV .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 155 (01) :270-277

[7] ROLE OF COBALT IN STABILIZING THE MOLECULAR-STRUCTURE OF GLUCOSE-ISOMERASE FROM STREPTOMYCES-GRISEOFUSCUS S-41 [J].

KASUMI, T ;

HAYASHI, K ;

TSUMURA, N .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1982, 46 (01) :31-39

[8] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[9]

Yamanaka K, 1975, Methods Enzymol, V41, P466

[10] STUDIES ON D-GLUCOSE ISOMERIZING ACTIVITY OF D-XYLOS GROWN CELLS FROM BACILLUS COAGULANS STRAIN HN-68 .I. DESCRIPTION OF STRAIN AND CONDITIONS FOR FORMATION OF ACTIVITY [J].

YOSHIMURA, S ;

DANNO, G ;

NATAKE, M .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1966, 30 (10) :1015-+

← 1 2 →