ARCHAEBACTERIAL LIPID MODELS - HIGHLY SALT-TOLERANT MEMBRANES FROM 1,2-DIPHYTANYLGLYCERO-3-PHOSPHOCHOLINE

被引：69

作者：

YAMAUCHI, K ^{[1
]}

DOI, K ^{[1
]}

KINOSHITA, M ^{[1
]}

KII, F ^{[1
]}

FUKUDA, H ^{[1
]}

机构：

[1] KANEKA CO,ENGN RES LABS,TAKASAGO,HYOGO,JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1110卷 / 02期

关键词：

LIPOSOME; ARCHAEBACTERIUM; HALOPHILE; PERMEABILITY; NMR; H-1-; FABMS;

D O I：

10.1016/0005-2736(92)90355-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1,2-Di(3RS,7R,11R-phytanyl)-sn-glycero-3-phosphocholine and its glycerol epimers were synthesized as model lipids of archaebacterial halophiles. These amphiphiles, upon sonication of aqueous suspensions, gave rise to small unilamellar vesicles (SUV) of 300-800 angstrom in diameter and about 80 angstrom in the membrane thickness. The liposomes were very stable for at least a month even in a highly concentrated suspension or 5 M aqueous NaCl. The vesicles could retain Na+ and Cl- ions as well as 5(6)-carboxyfluorescein in the aqueous interior at temperature as high as 70-degrees-C. The liposomes of ordinary diester lipids such as 1,2-dipalmitoyl-sn-glycero-3-phosphocholine and egg-yolk lecithin were less stable and more permeable than those of the diphytanyl lipids.

引用

页码：171 / 177

页数：7

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[1] HYPERFILTRATION THROUGH CROSSLINKED MONOLAYERS .2. [J].