AN IMPROVED METHOD FOR THE MEASUREMENT OF MALONDIALDEHYDE IN BIOLOGICAL SAMPLES

被引：82

作者：

LI, XY ^{[1
]}

CHOW, CK ^{[1
]}

机构：

[1] UNIV KENTUCKY,DEPT NUTR & FOOD SCI,LEXINGTON,KY 40506

来源：

LIPIDS | 1994年 / 29卷 / 01期

关键词：

D O I：

10.1007/BF02537094

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An improved method was developed for measuring malondialdehyde (MDA) as its thiobarbituric acid (TBA) complex. Samples were initially incubated with 1% potassium iodide and 0.1% butylated hydroxytoluene at 50 degrees C for 20 min, and then with 0.4% TBA at 60 degrees C for 60 min. The MDA-TBA complex formed was extracted with isobutyl alcohol and measured by high performance liquid chromatography with fluorescence detection. The improved method allows for a more specific determination of MDA present in biological samples.

引用

页码：73 / 75

页数：3

共 13 条

[1] ANALYSIS OF UNSATURATED FATTY-ACIDS, AND THEIR HYDROPEROXY AND HYDROXY DERIVATIVES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].