IMPROVED METHODS OF OLIGOSACCHARIDE ANALYSIS FOR GENETIC-STUDIES OF LEGUME SEEDS

被引：45

作者：

FRIAS, J

HEDLEY, CL

PRICE, KR

FENWICK, GR

VIDALVALVERDE, C

机构：

[1] JOHN INNES INST,DEPT APPL GENET,NORWICH NR2 7UH,ENGLAND

[2] INST FOOD RES,DEPT FOOD MOLEC BIOCHEM,NORWICH NR4 7UH,NORFOLK,ENGLAND

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1994年 / 17卷 / 11期

关键词：

D O I：

10.1080/10826079408013494

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The analysis of low molecular weight carbohydrates in single seed of lentils has been carried out using two different high performance liquid chromatography (HPLC) methods. One used a reversed-phase column coupled to a refractive index detector (HPLC-RI), while the other utilised an anion-exchange phase column coupled to a triple-pulsed amperometric detector (HPAC-PAD). The latter was found to be more sensitive and could be used for the analysis of very small samples, hence allowing parts of a seed to be analysed and then grown and used for genetic studies.

引用

页码：2469 / 2483

页数：15

共 26 条

[1]

ADSULE RN, 1989, CRC HDB WORLD LEGUME, V2, P132

[2]

ARENTOFT AM, 1992, 1ST P EUR C GRAIN LE, P457

[3] DETERMINATION, BY NMR-SPECTROSCOPY, OF THE STRUCTURE OF CICERITOL, A PSEUDOTRISACCHARIDE ISOLATED FROM LENTILS [J].