SEQUENCE AND FUNCTIONAL EXPRESSION OF AN AMPHIBIAN WATER CHANNEL, FA-CHIP - A NEW MEMBER OF THE MIP FAMILY

被引：58

作者：

ABRAMI, L ^{[1
]}

SIMON, M ^{[1
]}

ROUSSELET, G ^{[1
]}

BERTHONAUD, V ^{[1
]}

BUHLER, JM ^{[1
]}

RIPOCHE, P ^{[1
]}

机构：

[1] CEA SACLAY,DEPT CELLULAR & MOLEC BIOL,SERV BIOL CELLULAIRE,F-91191 GIF SUR YVETTE,FRANCE

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 1994年 / 1192卷 / 01期

关键词：

MAJOR INTRINSIC PROTEIN (MIP); CHANNEL FORMING INTEGRAL PROTEIN (CHIP28); OSMOTIC WATER PERMEABILITY; AMPHIBIAN URINARY BLADDER; RACE PCR; (R-ESCULENTA);

D O I：

10.1016/0005-2736(94)90155-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new member of the family of water channel proteins (aquaporin-CHIP) related to the major intrinsic protein (MIP) family is described. The cDNA coding for this amphibian CHIP vias cloned from frog (Rana esculenta) urinary bladder, a model for the kidney collecting duct, using a RT-PCR cloning strategy. The encoded protein, designated FA-CHIP (frog aquaporin-CHIP), shows 77.4%, 42.4% and 35.6% identity with the three proteins new referred to as the aquaporins of the MIP family, i.e., human CHIP28, WCH-CD and gamma-TIP, respectively. Xenopus leavis injected with FA-CHIP cRNA exhibited a marked increase of the osmotic water permeability.

引用

页码：147 / 151

页数：5

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