GENETIC DIVERSITY IN EUROPEAN AND MEDITERRANEAN FABA BEAN GERM PLASM REVEALED BY RAPD MARKERS

被引:225
作者
LINK, W
DIXKENS, C
SINGH, M
SCHWALL, M
MELCHINGER, AE
机构
[1] CCS HARYANA AGR UNIV, DEPT GENET, HISAR 125004, HARYANA, INDIA
[2] SUDWESTDEUTSCHE SAATZUCHT, D-76437 RASTATT, GERMANY
关键词
RAPD; GENETIC DIVERSITY; VICIA FABA L; GERM PLASM; GENE POOLS;
D O I
10.1007/BF00220992
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Broadening of the genetic base and systematic exploitation of heterosis in faba bean requires reliable information on the genetic diversity in the germ plasm. Three groups of faba bean inbred lines were examined by means of RAPDs (random amplified polymorphic DNAs) assays: 13 European small-seeded lines, 6 European large-seeded lines, and 9 Mediterranean lines. Out of 59 primers, 35 were informative and yielded 365 bands, 289 of which were polymorphic with a mean of 8.3 bands per primer. Monomorphic bands were omitted from the analyses and genetic distances (GD) were estimated via the coefficient of Jaccard. The mean GD among the European small-seeded lines was significantly greater than those among the lines of the other two groups. Repeatability of GD estimates was high. Cluster (UPGMA) and principal coordinate analyses identified European small-seeded lines and Mediterranean lines as distinct groups with European large-seeded lines located in between. The results are in harmony with published archaeobotanical findings. We conclude that RAPDs are useful for classification of germ plasm and identification of divergent heterotic groups in faba bean.
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页码:27 / 32
页数:6
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