KI-RAS AND C-MYC ONCOGENE EXPRESSION MEASURED BY COAMPLIFICATION POLYMERASE CHAIN-REACTION

被引：9

作者：

SUGIMOTO, T

TSUKAMOTO, F

FUJITA, M

TAKAI, S

机构：

[1] Department of Oncologic Surgery, School of Medicine, Osaka University, Osaka 565, 2-2 Yamada-Oka, Suita

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 201卷 / 02期

关键词：

D O I：

10.1006/bbrc.1994.1740

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ki-ras and c-myc oncogene mRNA in carcinoma tissue was quantitatively detected by the coamplification polymerase chain reaction. After the reverse transcription of the mRNA mixture with random hexanucleotide primer the coamplification polymerase chain reaction of the oncogene and the internal standard beta-actin cDNA was done. The amount of oncogene mRNA was calculated from the coamplified product ratio. Ki-ras expression in a human gastric cancer strain H-111 was estimated to 5 percent of beta-actin. The expression of c-myc mRNA in colorectal carcinoma tissue was observed to be ten to one hundred times higher than normal mucosa. Gene expression was compared numerically in mole-liter without using hybridization and radioactive probes. (C) 1994 Academic Press, Inc.

引用

页码：574 / 580

页数：7

共 18 条

[1] ABSOLUTE MESSENGER-RNA QUANTIFICATION USING THE POLYMERASE CHAIN-REACTION (PCR) - A NOVEL-APPROACH BY A PCR AIDED TRANSCRIPT TITRATION ASSAY (PATTY) [J].