ENGINEERING OF MICROHETEROGENEITY-RESISTANT PARA-HYDROXYBENZOATE HYDROXYLASE FROM PSEUDOMONAS-FLUORESCENS

被引：22

作者：

ESCHRICH, K

VANBERKEL, WJH

WESTPHAL, AH

DEKOK, A

MATTEVI, A

OBMOLOVA, G

KALK, KH

HOL, WGJ

机构：

[1] AGR UNIV WAGENINGEN,DEPT BIOCHEM,DREIJENLAAN 3,6703 HA WAGENINGEN,NETHERLANDS

[2] STATE UNIV GRONINGEN,DEPT CHEM,BISON RES INST,9747 AG GRONINGEN,NETHERLANDS

来源：

FEBS LETTERS | 1990年 / 277卷 / 1-2期

关键词：

PARA-HYDROXYBENZOATE HYDROXYLASE; PROTEIN-ENGINEERING; SITE-DIRECTED MUTAGENESIS; THIOL-REACTIVITY; CRYSTALLIZATION;

D O I：

10.1016/0014-5793(90)80843-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

By site-directed mutagenesis, Cys-116 was converted to Ser-116 in p-hydroxybenzoate hydroxylase (EC 1.14.13.2) from Pseudomonas fluorescens. In contrast to wild-type enzyme, the C116S mutant is no longer susceptible to oxidation by hydrogen peroxide and shows no reacitivity towards 5,5'-dithiobis(2-nitrobenzoate). Crystals of the C116S mutant are isomorphous with the crystal form of wild-type enzyme. A difference electron density confirms the mutation made.

引用

页码：197 / 199

页数：3

共 17 条

[1] PURIFICATION, PROPERTIES, AND OXYGEN REACTIVITY OF PARA-HYDROXYBENZOATE HYDROXYLASE FROM PSEUDOMONAS-AERUGINOSA [J].