IMMUNOAFFINITY PURIFICATION AND CDNA CLONING OF HUMAN PLATELET PROSTAGLANDIN ENDOPEROXIDE SYNTHASE (CYCLOOXYGENASE)

被引：41

作者：

TAKAHASHI, Y

UEDA, N

YOSHIMOTO, T

YAMAMOTO, S

YOKOYAMA, C

MIYATA, A

TANABE, T

FUSE, I

HATTORI, A

SHIBATA, A

机构：

[1] UNIV TOKUSHIMA,SCH MED,DEPT BIOCHEM,TOKUSHIMA 770,JAPAN

[2] NATL CARDIOVASC CTR,DEPT PHARMACOL,SUITA,OSAKA 565,JAPAN

[3] NIIGATA UNIV,SCH MED,DEPT INTERNAL MED 1,NIIGATA 951,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 182卷 / 02期

关键词：

D O I：

10.1016/0006-291X(92)91750-K

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cDNA for prostaglandin endoperoxide synthase (cyclooxygenase) was cloned from human platelets by the polymerase chain reaction amplification method, and the primary structure of the enzyme was deduced from the nucleotide sequence. The enzyme was composed of 599 amino acids including 23-amino acid signal sequence, and the calculated molecular weight of the mature protein was 65,995. The enzyme was immunoaffinity-purified from human platelets. The N-terminal amino acid sequence determined by Edman degradation was Ala-Asp-Pro-Gly-Ala-Pro-Thr-Pro-, and the result confirmed the primary structure of the enzyme, which was deduced from the cDNA sequence. © 1992.

引用

页码：433 / 438

页数：6

共 21 条

[1] CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
[2] PRIMARY STRUCTURE OF PROSTAGLANDIN-G/H SYNTHASE FROM SHEEP VESICULAR GLAND DETERMINED FROM THE COMPLEMENTARY-DNA SEQUENCE
DEWITT, DL
SMITH, WL
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (05) : 1412 - 1416
[3] DEWITT DL, 1990, J BIOL CHEM, V265, P5192
[4] EHARA H, 1988, BIOCHIM BIOPHYS ACTA, V960, P35
[5] HUMAN PLATELET ERYTHROLEUKEMIA CELL PROSTAGLANDIN G/H SYNTHASE - CDNA CLONING, EXPRESSION, AND GENE CHROMOSOMAL ASSIGNMENT
FUNK, CD
FUNK, LB
KENNEDY, ME
PONG, AS
FITZGERALD, GA
[J]. FASEB JOURNAL, 1991, 5 (09) : 2304 - 2312
[6] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4
LAEMMLI, UK
[J]. NATURE, 1970, 227 (5259) : 680 - +
[7] MERLIE JP, 1988, J BIOL CHEM, V263, P3550
[8] MIYAMOTO T, 1976, J BIOL CHEM, V251, P2629
[9] MUTSAERS JHGM, 1985, EUR J BIOCHEM, V147, P569
[10] NAKANISHI K, 1984, SCAND J HAEMATOL, V32, P167

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