DNA degradation and proteolysis in thymocyte apoptosis

被引：19

作者：

Fearnhead, HO ^{[1
]}

MacFarlane, M ^{[1
]}

Dinsdale, D ^{[1
]}

Cohen, GM ^{[1
]}

机构：

[1] UNIV LEICESTER, MRC, TOXICOL UNIT, LEICESTER LE1 9HN, LEICS, ENGLAND

来源：

TOXICOLOGY LETTERS | 1995年 / 82-3卷

关键词：

thymocyte apoptosis; TLCK; proteases; interleukin-1; beta;

D O I：

10.1016/0378-4274(95)03473-0

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

Data from a number of model systems support a role for proteolysis in apoptotic cell death. Using immature rat thymocytes, we demonstrate that the protease inhibitors N-alpha-tosyl-L-lysinyl-chloromethylketone (TLCK) and benzyloxycarbonyl-valinyl-alaninyl-aspartyl fluoromethylketone (Z-VAD.FMK) inhibit apoptosis. N-tosyl-L-phenylalaninyl-chloromethylketone (TPCK) has a very different effect, inducing the early morphological and biochemical changes associated with apoptosis. TLCK inhibits trypsin-like proteases whilst Z-VAD.FMK inhibits interleukin-1 beta-converting enzyme (ICE)-like proteases; this and the contrasting effects of TPCK support the hypothesis that thymocyte apoptosis involves a hierarchy of proteases which act at different stages of the process.

引用

页码：135 / 141

页数：7

共 22 条

[1]

ARENDS MJ, 1991, INT REV EXP PATHOL, V32, P223

[2]

BROWN DG, 1993, J BIOL CHEM, V268, P3037

[3]

BRUNO S, 1992, LEUKEMIA, V6, P1113

[4]

COHEN GM, 1994, J IMMUNOL, V153, P507

[5] KEY MORPHOLOGICAL FEATURES OF APOPTOSIS MAY OCCUR IN THE ABSENCE OF INTERNUCLEOSOMAL DNA FRAGMENTATION [J].

COHEN, GM ;

SUN, XM ;

SNOWDEN, RT ;

DINSDALE, D ;

SKILLETER, DN .

BIOCHEMICAL JOURNAL, 1992, 286 :331-334

[6] APOPTOSIS AND PROGRAMMED CELL-DEATH IN IMMUNITY [J].

COHEN, JJ ;

DUKE, RC ;

FADOK, VA ;

SELLINS, KS .

ANNUAL REVIEW OF IMMUNOLOGY, 1992, 10 :267-293

[7] A PREEXISTING PROTEASE IS A COMMON EFFECTOR OF THYMOCYTE APOPTOSIS MEDIATED BY DIVERSE STIMULI [J].

FEARNHEAD, HO ;

RIVETT, AJ ;

DINSDALE, D ;

COHEN, GM .

FEBS LETTERS, 1995, 357 (03) :242-246

[8]

Kerr J.F.R., 1987, P93

[9] ICE-LIKE PROTEASES IN APOPTOSIS [J].

KUMAR, S .

TRENDS IN BIOCHEMICAL SCIENCES, 1995, 20 (05) :198-202

[10] INDUCTION OF APOPTOSIS IN FIBROBLASTS BY IL-1-BETA-CONVERTING ENZYME, A MAMMALIAN HOMOLOG OF THE C-ELEGANS CELL-DEATH GENE CED-3 [J].

MIURA, M ;

ZHU, H ;

ROTELLO, R ;

HARTWIEG, EA ;

YUAN, JY .

CELL, 1993, 75 (04) :653-660

← 1 2 3 →