槲皮素对四氯化碳引起的小鼠急性肝损伤治疗作用及其机理(英文)

被引：37

作者：

Jiaqi ZHANG ^{[1
,2
]}

Liang SHI ^{[1
,2
]}

Xining XU ^{[2
]}

Sichong HUANG ^{[2
]}

Bin LU ^{[1
]}

Lili JI ^{[1
]}

Zhengtao WANG ^{[1
]}

机构：

[1] MOE Key Laboratory for Standardization of Chinese Medicines and Shanghai Key Laboratory of Complex Prescription,Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine

[2] School of Pharmacy, Shanghai University of Traditional Chinese Medicine

来源：

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) | 2014年 / 15卷 / 12期

关键词：

肝毒性; 氧应激损伤; Prx; Nrf2; Trx Rd; Trx; HO-1;

D O I：

暂无

中图分类号：

R285.5 [中药实验药理];

学科分类号：

100806 [中药药理学];

摘要：

研究目的:本研究旨在观察槲皮素对四氯化碳(CCl4)诱导的肝损伤的治疗解毒作用及其机理。创新要点:首次发现槲皮素对CCl4诱导的肝损伤有治疗作用,并且首次发现Prx和Trx家族参与其中。研究方法 :检测小鼠血清转氨酶含量,并检测肝组织中丙二醛(MDA)、谷胱甘肽(GSH)和4-羟基壬烯醛(4-HNE)含量,并用实时聚合酶链式反应(PCR)检测肝组织中Prx 1–6、Trx R1/2、Trx1/2、Nrf2和HO-1的mR NA表达情况。重要结论:CCl4造模成功后口服槲皮素对其造成的急性肝损伤有治疗作用,给药组小鼠血清中的转氨酶与模型组相比均有显著下降,通过MDA和免疫组化分析其机理可能和保护氧化应激损伤有关,通过实时PCR分析发现CCl4抑制了抗氧化酶Prx家族、Trx Rd1、TrxR d2、Trx1、Trx2和Nrf2及其下游HO-1的基因表达,而槲皮素可以逆转CCl4降低的这些基因的表达。

引用

页码：1039 / 1047

页数：9

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