没药甾酮对HO损伤PC12细胞的保护作用（英文）

被引：19

作者：

徐宏彬 ^{[1
]}

李玲 ^{[1
]}

刘国卿 ^{[2
]}

机构：

[1] 同济大学附属第十人民医院药剂科

[2] 中国药科大学药理学教研室

来源：

药学学报 | 2008年 / 12期

关键词：

没药甾酮; PC12细胞; 氧化应激; 过氧化氢;

D O I：

暂无

中图分类号：

R285 [中药药理学];

学科分类号：

100806 [中药药理学];

摘要：

探讨没药甾酮(guggulsterone)对氧化应激损伤PC12细胞的保护作用。以过氧化氢(hydrogen peroxide,H2O2)损伤PC12细胞为氧化应激损伤模型,维生素E为对照,采用四甲基偶氮唑蓝[3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl-tetrazolium bromide,MTT]法检测细胞增殖状况;试剂盒检测乳酸脱氢酶(lactate dehydrogenase,LDH)及一氧化氮(nitric oxide,NO)的释放;DCFH法和Fura 2-AM法检测细胞内活性氧(reactive oxygen species,ROS)和Ca2+的含量;碘化丙啶(propidium iodide,PI)染色流式细胞术(flow cytometry,FCM)检测细胞凋亡;罗丹明123(rhodamine 123,Rh 123)染色FCM检测细胞线粒体膜电位(mitochondrial membrane protential,MMP)。结果表明,没药甾酮(0.1～10μmol.L-1)可使200μmol.L-1H2O2作用24 h后的PC12细胞生长抑制率下降;细胞外LDH和NO,细胞内ROS和Ca2+含量降低;明显抑制200μmol.L-1H2O2作用12 h后诱导的PC12细胞凋亡和线粒体膜电位降低作用,没药甾酮(0.1～10μmol.L-1)使细胞凋亡率由24.3%下降至18.4%、15.9%、11.8%。实验结果表明,没药甾酮对氧化应激损伤PC12细胞具有保护作用,其机制可能为降低细胞内ROS含量,进而抑制LDH和NO释放,降低细胞内Ca2+含量,升高线粒体膜电位,减少细胞凋亡。

引用

页码：1190 / 1197

页数：8

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