骨髓间充质干细胞定向分化为血管内皮样细胞的实验研究附视频

被引：3

作者：

徐蓉 ^{[1
]}

徐金勇 ^{[2
]}

刘玮 ^{[1
]}

机构：

[1] 安徽医学高等专科学校药学系

[2] 安徽医科大学基础医学院人体解剖学教研室

来源：

生物医学工程学杂志 | 2014年 / 02期

关键词：

骨髓间充质干细胞; 血管内皮样细胞; 培养; 分化;

D O I：

暂无

中图分类号：

R329 [人体组织学];

学科分类号：

摘要：

体外诱导人骨髓的间充质干细胞(MSCs)分化成为血管内皮样细胞(ELCs),探讨骨髓来源的MSCs作为组织工程血管种子细胞的可行性。通过密度梯度离心,分离人骨髓的单个核细胞,DMEM贴壁培养MSCs,多次传代贴壁培养纯化MSCs,包含血管内皮生长因子(VEGF)、人成纤维细胞生长因子(hFGF)、胰岛素样生长因子-1(IGF-1)和人表皮生长因子(hEGF)的EBM-2培养基诱导MSCs向ELCs分化,倒置显微镜观察ELCs的形态,流式细胞仪检测ELCs的血管内皮钙粘素(VE-cadherin)、CD133和CD31表达,免疫荧光检测ELCs的CD31和血管性假血友病因子(vWF)表达。结果显示,贴壁培养的MSCs呈长梭形,旋涡状生长,经过诱导细胞形态发生改变,细胞形态变圆,与血管内皮细胞(ECs)相似;流式细胞仪结果显示ELCs表达ECs的特异性标志物CD31和VE-cadherin,不表达CD133;免疫荧光结果也显示ELCs表达CD31和vWF。结果表明:MSCs具有定向诱导分化为ECs的潜能,MSCs来源的ELCs具有与ECs相似的细胞生物学特征,提示骨髓来源的MSCs可作为血管组织工程的种子细胞。

引用

收藏

页码：389 / 393

页数：5

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共 8 条

[1] Adipose Tissue-Derived Stem Cell in Vitro Differentiation in a Three-Dimensional Dental Bud Structure
Ferro, Federico
Spelat, Renza
Falini, Giuseppe
Gallelli, Annarita
D'Aurizio, Federica
Puppato, Elisa
Pandolfi, Maura
Beltrami, Antonio Paolo
Cesselli, Daniela
Beltrami, Carlo Alberto
Ambesi-Impiombato, Francesco Saverio
Curcio, Francesco
[J]. AMERICAN JOURNAL OF PATHOLOGY, 2011, 178 (05) : 2299 - 2310
[2] Fibrin microthreads support mesenchymal stem cell growth while maintaining differentiation potential[J] . Megan K.Proulx,Shawn P.Carey,Lisa M.DiTroia,Craig M.Jones,MichaelFakharzadeh,Jacques P.Guyette,Amanda L.Clement,Robert G.Orr,Marsha W.Rolle,George D.Pins,Glenn R.Gaudette. J. Biomed. Mater. Res. . 2010 (2)
[3] Behavior of Human Mesenchymal Stem Cells in Fibrin-Based Vascular Tissue Engineering Constructs
O'Cearbhaill, Eoin D.
Murphy, Mary
Barry, Frank
McHugh, Peter E.
Barron, Valerie
[J]. ANNALS OF BIOMEDICAL ENGINEERING, 2010, 38 (03) : 649 - 657
[4] Preliminary Investigation of Seeding Mesenchymal Stem Cells on Biodegradable Scaffolds for Vascular Tissue Engineering In Vitro
Li, Chun-Min
Wang, Zhong-Gao
Gu, Yong-Quan
Dong, Jian-De
Qiu, Rong-Xin
Bian, Ce
Liu, Xiao-Fei
Feng, Zeng-Guo
[J]. ASAIO JOURNAL, 2009, 55 (06) : 614 - 619
[5] Endothelial Differentiation Potential of Human Monocyte‐Derived Multipotential Cells[J] . MasatakaKuwana,YukaOkazaki,HiroakiKodama,TakashiSatoh,YutakaKawakami,YasuoIkeda. STEM CELLS . 2009 (12)
[6] Application of stem cells for cardiovascular grafts tissue engineering
Wu, Kaihong
Liu, Ying Long
Cui, Bin
Han, Zhongchao
[J]. TRANSPLANT IMMUNOLOGY, 2006, 16 (01) : 1 - 7
[7] Advancing vascular tissue engineering: the role of stem cell technology[J] . Kevin M. Sales,Henryk J. Salacinski,Nasser Alobaid,Michael Mikhail,Vishni Balakrishnan,Alexander M. Seifalian. Trends in Biotechnology . 2005 (9)
[8] Therapeutic angiogenesis for patients with limb ischaemia by autologous transplantation of bone-marrow cells: a pilot study and a randomised controlled trial[J] . Eriko Tateishi-Yuyama,Hiroaki Matsubara,Toyoaki Murohara,Uichi Ikeda,Satoshi Shintani,Hiroya Masaki,Katsuya Amano,Yuji Kishimoto,Kohji Yoshimoto,Hidetoshi Akashi,Kazuyuki Shimada,Toshiji Iwasaka,Tsutomu Imaizumi. The Lancet . 2002 (9331)