侵袭性真菌病的非培养实验室诊断方法

被引:11
作者
杨启文
徐英春
机构
[1] 中国医学科学院北京协和医学院北京协和医院检验科
关键词
真菌病; 抗原,真菌; 抗体,真菌; 血清学试验; 核酸扩增技术;
D O I
暂无
中图分类号
R519 [真菌与放线菌传染病]; R446.6 [免疫学检验];
学科分类号
100401 ; 100208 ;
摘要
侵袭性真菌病的非培养实验室检测方法主要包括真菌抗原检测、真菌抗体血清学检测以及分子生物学检测。真菌抗原检测包括(1,3)-β-D-葡聚糖检测、半乳甘露聚糖检测、隐球菌荚膜多糖抗原检测以及念珠菌甘露聚糖抗原检测等。不同的抗原用于诊断不同的真菌感染。抗真菌抗体的血清学检测主要包括荚膜组织胞浆菌、粗球孢子菌/副球孢子菌以及皮炎芽生菌的相应血清抗体的检测。分子生物学检测则包括非扩增的核酸检测技术、核酸扩增检测技术、直接测序以及基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)技术。尽管分子生物学检测的方法尚未标准化,但其是未来侵袭性真菌病非培养检测的重要发展方向。
引用
收藏
页码:721 / 724
相关论文
共 5 条
[1]  
Evaluation of the peptide nucleic acid fluorescence in situ hybridisation technology for yeast identification directly from positive blood cultures: an Italian experience[J] . Claudio Farina,Silvana Perin,Stefano Andreoni,Marco Conte,Paolo Fazii,Gianluigi Lombardi,Esther Manso,Cristina Morazzoni,Silvana Sanna.Mycoses . 2012 (5)
[2]  
Diagnosis of candidemia by polymerase chain reaction and blood culture: prospective study in a high-risk population and identification of variables associated with development of candidemia[J] . M. S. Moreira-Oliveira,Y. Mikami,M. Miyaji,T. Imai,A. Z. Schreiber,M. L. Moretti.European Journal of Clinical Microbiology & Infectious Diseases . 2005 (11)
[3]   Polymerase chain reaction screening for fungemia and/or invasive fungal infections in patients with hematologic malignancies [J].
Ribeiro, P ;
Costa, F ;
Monteiro, A ;
Caldas, J ;
Silva, M ;
Ferreira, G ;
Veiga, J ;
Sousa, MO ;
Viegas, MP ;
Santos, E ;
Gonçalves, AJ ;
Sousa, AB .
SUPPORTIVE CARE IN CANCER, 2006, 14 (05) :469-474
[4]   Contribution of serological tests and blood culture to the early diagnosis of systemic candidiasis [J].
Yera, H ;
Sendid, B ;
Francois, N ;
Camus, D ;
Poulain, D .
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2001, 20 (12) :864-870
[5]  
Detection of Aspergillus Species in Blood and Bronchoalveolar Lavage Samples from Immunocompromised Patients by Means of 2-Step Polymerase Chain Reaction: Clinical Results .2 Dieter Buchheidt,Corinna Baust,Heyko Skladny,Joerg Ritter,Thomas Suedhoff,Michael Baldus,Wolfgang Seifarth,Christine Leib-Moesch,Ruediger Hehlma. Clinical Infectious Diseases . 2001