高表达转C4型PEPC基因水稻在低氮下诱导碳氮酶稳定光合作用

被引：7

作者：

唐玉婷 ^{[1
,2
]}

李霞 ^{[2
]}

陆巍 ^{[1
]}

魏晓东 ^{[2
]}

机构：

[1] 南京农业大学生命科学学院

[2] 江苏省农业科学院粮食作物研究所江苏省优质水稻工程技术研究中心国家水稻改良中心南京分中心

来源：

华北农学报 | 2015年 / 30卷 / 04期

关键词：

高表达转C4型PEPC基因水稻; 低氮; 光合特性;

D O I：

暂无

中图分类号：

S511 [稻];

学科分类号：

摘要：

为揭示高表达转玉米C4-PEPC基因水稻在低氮条件下的光合表现,采用高表达转玉米C4-PEPC基因水稻(PC)与原种Kitaake(WT)为试验材料,通过盆栽试验,分别测定不同的氮素条件下(中氮300 kg/hm2,低氮65 kg/hm2),开花后不同天数的SPAD值,光合参数以及碳氮关键酶活性。并在开花后50 d收获植株,记录产量因子。结果表明:低氮处理下的PC相对于WT来说,在开花后14,28 d,其净光合速率分别提高了13.10%(P<0.05)和29.29%(P<0.05)。同时,Rubisco羧化酶在开花后14,28 d时活性分别提高了67.86%和52.63%(P<0.05);硝酸还原酶在14,28 d时的活性分别提高79.49%(P<0.05)和17.96%;谷氨酰胺合成酶仅在开花后28 d时活性提高28.48%(P<0.05)。但是通过对产量进行分析,在低氮条件下,并未发现PC的产量与WT有明显差异。可见,PC通过诱导碳氮的关键酶活性的提高维持低氮条件下高净光合速率。

引用

页码：95 / 100

页数：6

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