CRISPR-Cas9介导的基因组编辑技术的研究进展附视频

被引：24

作者：

郑小梅 ^{[1
,2
]}

张晓立 ^{[1
,3
]}

于建东 ^{[1
,2
]}

郑平 ^{[1
,2
]}

孙际宾 ^{[1
,2
]}

机构：

[1] 中国科学院系统微生物工程重点实验室

[2] 中国科学院天津工业生物技术研究所

[3] 天津科技大学生物工程学院

来源：

生物技术进展 | 2015年 / 01期

关键词：

CRISPR-Cas系统; Cas9; 基因组编辑技术;

D O I：

暂无

中图分类号：

Q811.4 [生物信息论];

学科分类号：

0711 ; 0831 ;

摘要：

CRISPR-Cas(clustered regularly interspaced short palindromic repeats-CRISPR-associated proteins)系统为细菌与古生菌中抵御外源病毒或质粒DNA入侵的获得性免疫系统。该系统在crRNA的指导下,使核酸酶Cas识别并降解外源DNA。其中,Ⅱ型CRISPR-Cas系统最为简单,仅包括一个核酸酶Cas9与tracrRNA:crRNA二聚体便可完成其生物功能。基于CRISPR-Cas9的基因组编辑技术的核心为将tracrRNA:crRNA设计为引导RNA,在引导RNA的指导下Cas9定位于特定DNA序列上,进行DNA双链切割,实现基因组的定向编辑。CRISPR-Cas9系统以设计操纵简便、编辑高效与通用性广等优势成为新一代基因组编辑技术,为基因组定向改造调控与应用等带来突破性革命。从CRISPR-Cas9介导的基因组编辑技术的发展与应用等方面综述其最新研究进展,并着重介绍该技术的关键影响因素,为相关研究者提供参考。

引用

页码：1 / 9+78 +78-79

页数：11

共 14 条

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