东亚钳蝎毒素多肽对河豚毒素敏感型钠电流的作用

被引：3

作者：

安珊珊 ^{[1
]}

汪溪洁 ^{[1
]}

许鹏 ^{[1
]}

孙芹 ^{[1
]}

陈晓东 ^{[2
]}

程洁 ^{[1
]}

高蓉 ^{[1
]}

肖杭 ^{[1
]}

机构：

[1] 南京医科大学现代毒理学教育部重点实验室江苏省医学动物实验基地

[2] 徐州医学院麻醉学院

来源：

医学研究生学报 | 2009年 / 22卷 / 06期

关键词：

东亚钳蝎; 蝎毒; 河豚毒素敏感型钠通道电流; 膜片钳;

D O I：

暂无

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

目的:蝎毒素是一种神经毒素,其对周围神经系统钠通道的作用尚不十分清楚,仍有许多疑题待研究。文中研究东亚钳蝎毒素多肽224(BmK224)对大鼠背根神经节(DRG)神经元电压依赖性河豚毒素敏感型钠电流(TTX-S INa)的影响。方法:分离单个DRG神经元,应用全细胞膜片钳技术观察BmK224对TTX-S INa的影响。结果:BmK224浓度依赖性地抑制TTX-S INa,40μg/m lBmK224使TTX-S INa稳态激活和失活曲线都向超极化方向移动,复活时间延长,可激活通道数增加。结论:BmK224影响钠通道激活失活过程的作用机制可能是所含的α-蝎毒素使钠通道构象发生改变。

引用

页码：564 / 569

页数：6

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