山奈酚对胃癌细胞PARP1以及P53基因表达的影响研究附视频

被引:53
作者
王丽君
戴志升
机构
[1] 海南省第二人民医院
关键词
胃癌; 山奈酚; P53; PARP1;
D O I
暂无
中图分类号
R285.5 [中药实验药理];
学科分类号
100806 [中药药理学];
摘要
胃癌(GC)是最常见的恶性肿瘤之一,是人类健康的主要威胁,其发病机制是一个单基因或多基因逐步突变的过程,与细胞的侵袭、增殖和转移有关,包括癌基因遗传和表观遗传的突变、肿瘤抑制基因、DNA修复途径基因、细胞周期途径基因和幽门螺杆菌感染等。而山奈酚具有多种生物学活性,能够抑制多种肿瘤细胞的细胞周期,诱导肿瘤细胞凋亡从而抑制肿瘤细胞/组织的侵袭及转移。因此本研究用不同浓度的山奈酚处理胃癌细胞,并检测了胃癌细胞的形态变化情况、癌细胞凋亡相关因子P53和PARP1基因的表达水平和其对应的蛋白质表达变化。结果表明大于100μmol/L山奈酚处理后的胃癌细胞中P53基因和P53蛋白的表达水平被显著提高,而相反的PARP1基因和蛋白的表达则被显著抑制,且山奈酚处理后胃癌细胞的凋亡数目也明显增加,因此本实验结果表明,山奈酚能够有效的促进胃癌细胞凋亡的发生,以此来达到抑制癌细胞恶性增殖的作用。这一结果可以为后续针对胃癌新疗法的研究提供一些思路和理论支持。
引用
收藏
页码:1270 / 1274
页数:5
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[1]
Carcinoma of the stomach: A review of epidemiology; pathogenesis; molecular genetics and chemoprevention[J] Siddavaram Nagini; World Journal of Gastrointestinal Oncology 2012,
[2]
Kaempferol Suppresses Transforming Growth Factor-β1–Induced Epithelial-to-Mesenchymal Transition and Migration of A549 Lung Cancer Cells by Inhibiting Akt1-Mediated Phosphorylation of Smad3 at Threonine-179[J] Eunji Jo;Seong Ji Park;Yu Sun Choi;Woo-Kwang Jeon;Byung-Chul Kim Neoplasia 2015,
[3]
Cancer incidence and mortality worldwide: Sources; methods and major patterns in GLOBOCAN 2012[J] Jacques Ferlay;Isabelle Soerjomataram;Rajesh Dikshit;Sultan Eser;Colin Mathers;Marise Rebelo;Donald Maxwell Parkin;David Forman;Freddie Bray Int. J. Cancer 2015,
[4]
microRNA-9 suppresses the proliferation; invasion and metastasis of gastric cancer cells through targeting cyclin D1 and Ets1.[J] Zheng Liduan;Qi Teng;Yang Dehua;Qi Meng;Li Dan;Xiang Xuan;Huang Kai;Tong Qiangsong PloS one 2013,
[5]
The impact of post-transcriptional regulation in the p53 network[J] Justin A. Freeman;Joaquin M. Espinosa Briefings in Functional Genomics 2013,
[6]
Pre-clinical and clinical evaluation of PARP inhibitors as tumour-specific radiosensitisers[J] C. Powell;C. Mikropoulos;S.B. Kaye;C.M. Nutting;S.A. Bhide;K. Newbold;K.J. Harrington Cancer Treatment Reviews 2010,
[7]
NAD(P)H:quinone oxidoreductase 1 NQO1*2 genotype (P187S) is a strong prognostic and predictive factor in breast cancer.[J] Fagerholm Rainer;Hofstetter Barbara;Tommiska Johanna;Aaltonen Kirsimari;Vrtel Radek;Syrjäkoski Kirsi;Kallioniemi Anne;Kilpivaara Outi;Mannermaa Arto;Kosma Veli-Matti;Uusitupa Matti;Eskelinen Matti;Kataja Vesa;Aittomäki Kristiina;von Smitten Karl;Heikkilä Päivi;Lukas Jiri;Holli Kaija;Bartkova Jirina;Blomqvist Carl;Bartek Jiri;Nevanlinna Heli Nature genetics 2008,
[8]
Intercellular transfer of the oncogenic receptor EGFRvIII by microvesicles derived from tumour cells.[J] Al-Nedawi Khalid;Meehan Brian;Micallef Johann;Lhotak Vladimir;May Linda;Guha Abhijit;Rak Janusz Nature cell biology 2008,
[9]
The PARP superfamily. Amé Jean-Christophe;Spenlehauer Catherine;de Murcia Gilbert BioEssays : news and reviews in molecular; cellular and developmental biology 2004,
[10]
The role of photobiomodulation on gene expression of cell adhesionmolecules in diabetic wounded fibroblasts in vitro Ayuk SM;Abrahamse H;Houreld NN; J Photochem Photobiol B 2016,