海马注射β-淀粉样蛋白前体蛋白抗体诱导神经元的退行性变及学习记忆障碍(英文)附视频

被引:17
作者
许玉霞
王洪权
赵红
郭景春
朱粹青
机构
[1] 复旦大学上海医学院医学神经生物学国家重点实验室
关键词
淀粉样蛋白前体蛋白; 水迷宫; 神经元退行性变; 海马; paxillin; tau;
D O I
暂无
中图分类号
R749.16 [];
学科分类号
摘要
探讨海马内注射淀粉样蛋白前体蛋白(APP)抗体诱导细胞表面APP的铰链是否影响大鼠的水迷宫行为学以及是否诱导神经元的退行性改变,并进一步探讨其可能的机制.成年雄性SD大鼠海马内分别注射生理盐水、对照IgG和APP抗体.水迷宫行为学检测测试动物的学习和记忆能力.Cresyl Violet(CV)和Fluoro-Jade B染色观察神经元的退行性变.免疫组织化学方法检测MAP-2和磷酸化paxillin及磷酸化tau蛋白在海马的异常表达和分布.海马内注射APP抗体可延长动物的寻台潜伏期,减少大鼠在平台所在象限的探索时间和穿梭次数.CV和Fluoro Jade-B染色结果显示,海马注射APP抗体可导致海马锥体细胞的死亡和退变.同时伴MAP2免疫染色的减少和磷酸化paxillin及磷酸化tau的免疫染色的增加.上述结果表明,海马内注射APP抗体可诱导学习和记忆功能障碍及神经元的退行性改变,其机制可能与MAP-2和磷酸化paxillin及磷酸化tau的异常表达分布有关.
引用
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页码:908 / 918
页数:11
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