普通小麦-提莫菲维小麦白粉病抗性渐渗系中渗入片段的准确鉴定

被引：8

作者：

陶文静

刘金元

刘大钧

陈佩度

机构：

[1] 中国农业科学院生物技术研究中心!北京,山东省农业科学院原子能研究所!济南,南京农业大学农业部细胞遗传实验室!南京,南京农业大学农业部细胞遗传实验室!南京

来源：

植物学报 | 1999年 / 09期

关键词：

普通小麦; 提莫菲维小麦; Pm6渐渗系; 易位断点; RFLP标记;

D O I：

暂无

中图分类号：

S512.103 [];

学科分类号：

摘要：

用小麦（ＴｒｉｔｉｃｕｍａｅｓｔｉｖｕｍＬ．）第二部分同源群的３６个探针，对携带抗白粉病（Ｅｒｙｓｉｐｈｅｇｒａｍｉｎｉｓｆ．ｓｐｔｒｉｔｉｃｉ）基因Ｐｍ６的普通小麦提莫菲维小麦（Ｔ．ｔｉｍｏｐｈｅｅｖｉＺｈｕｋ．）渐渗系进行检测，通过这些渐渗系与受体亲本“Ｐｒｉｎｓ”之间杂交谱带多态性的比较，发现所测出多态性标记位点均位于这５个渐渗系的２Ｂ染色体上，但其渗入片段的位置与大小有明显区别。ＩＧＶ１＿４５６和ＩＧＶ１＿４５８被鉴定为２Ｂ染色体从短臂标记Ｘｃｄｏ４０５到长臂标记Ｘｂｃｄ１３５之间的区域已被提莫菲维２Ｇ染色体区段所代替；而ＩＧＶ１＿４６３则在２Ｂ染色体长臂Ｘｂｃｄ３０７到Ｘｃｄｏ６７８标记之间的区域被提莫菲维渗入成分所代替；ＩＧＶ１＿４６４、ＩＧＶ１＿４６５２ＢＬ染色体上的渗入片段更小，即ＩＧＶ１＿４６４在２ＢＬ染色体上标记Ｘｐｓｒ９３４与Ｘｂｃｄ１３５之间的区域有提莫菲维２Ｇ染色质成分渗入，ＩＧＶ１＿４６５仅在Ｘｂｃｄ１３５附近有更小的外源成分渗入。根据５个渐渗系渗入成分重叠区段比较可把Ｐｍ６定位于２ＢＬ染色体上Ｘｂｃｄ１３５２ＢＬ标记的邻近区域内

引用

页码：941 / 946

页数：6

共 9 条

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