猕猴桃属种间体细胞杂种(英文)

被引：10

作者：

肖尊安

韩碧文

机构：

[1] 北京师范大学生物系

[2] 中国农业大学生物学院北京

[3] 北京

来源：

ActaBotanicaSinica | 1997年 / 12期

关键词：

中华猕猴桃; 美味猕猴桃; 狗枣猕猴桃; 种间体细胞杂种;

D O I：

暂无

中图分类号：

Q943 [植物细胞遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

利用PEG融合方法,分别进行了中华猕猴桃(Actinidia chinensis var.chinensis)(2n=2x=58)子叶愈伤组织来源的原生质体与美味猕猴桃(A.deliciosa var.deiciosa)(2n=6x=174)子叶愈伤组织原生质体、以及狗枣猕猴桃(A.kolomikta)(2n=2x=58)叶肉原生质体种间原生质体融合。结果表明:中华猕猴桃与美味猕猴桃融合的1个克隆和中华猕猴桃与狗枣猕猴桃融合的4个克隆的RAPD谱带分别具有双亲特异的DNA谱带;经流式细胞仪分析,前者细胞核倍性推测为8倍体,后者细胞核为3倍体、4倍体和5倍体。初步鉴定这5个克隆是猕猴桃属种间体细胞杂种。

引用

页码：1110 / 1117

页数：8

共 11 条

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