骨髓间充质干细胞立体定向移植治疗大鼠脊髓损伤

被引:9
作者
管雅琳 [1 ]
孔繁明 [2 ]
王世民 [3 ]
吴盛 [3 ]
汪皖君 [3 ]
唐帆 [2 ]
张文治 [4 ]
机构
[1] 天津医科大学研究生院
[2] 天津市环湖医院病理科
[3] 天津市环湖医院神经内科
[4] 天津市环湖医院神经外科研究所细胞室
关键词
脊髓损伤; 骨髓间充质干细胞; 立体定向; 细胞移植; 大鼠;
D O I
暂无
中图分类号
R651.2 [脊髓];
学科分类号
摘要
背景:干细胞治疗神经组织损伤的关键在于移植具有再生能力的干细胞,通过多种作用机制,重建中枢神经系统的结构和功能。目的:立体定向移植骨髓间充质干细胞观察其对大鼠脊髓损伤修复的影响并探讨其机制。方法:密度梯度离心法结合贴壁筛选法分离骨髓间充质干细胞,于细胞移植前掺入10mg/L的BrdU进行标记。将成年雌性Wistar大鼠以动脉瘤夹闭法制备大鼠脊髓损伤模型后随机分为对照组、生理盐水组与移植组。移植组大鼠致伤后第7天,通过立体定向途径移植骨髓间充质干细胞到脊髓损伤中心,生理盐水组给予等量生理盐水,对照组大鼠不做处理。于大鼠脊髓损伤前及损伤后第7,14,30,60,90天进行BBB评分;损伤后第90天处死大鼠,观察其脊髓组织中有无BrdU阳性细胞、Brdu+神经元特异性烯醇化酶、Brdu+胶质纤维酸性蛋白、Brdu+碱性成纤维细胞生长因子、Brdu+脑源性神经生长因子免疫组织化学双染阳性细胞及单染阳性细胞。结果与结论:①骨髓间充质干细胞移植组大鼠BBB后肢功能评分恢复优于对照组(P<0.05);生理盐水组BBB评分在损伤后30d内恢复速度慢于对照组(P<0.05),至第90天与对照组比较差异无显著性意义(P>0.05)。②移植组大鼠脊髓内在损伤中心及头、尾端距离脊髓损伤中心1cm处均可见BrdU染色阳性细胞及免疫组织化学双染阳性细胞。移植组神经元特异性烯醇化酶、胶质纤维酸性蛋白、碱性成纤维细胞生长因子、脑源性神经生长因子单染阳性细胞数明显高于对照组和生理盐水组(P<0.05)。结果提示,骨髓间充质干细胞移植可以促进脊髓损伤大鼠的神经功能的恢复,其机制可能与移植细胞分化为神经元样和神经胶质细胞样细胞,并分泌或促进宿主分泌神经营养因子有关。
引用
收藏
页码:2549 / 2555
页数:7
相关论文
共 14 条
[1]   Long-term observation of auto-cell transplantation in non-human primate reveals safety and efficiency of bone marrow stromal cell-derived Schwann cells in peripheral nerve regeneration [J].
Wakao, Shohei ;
Hayashi, Takuya ;
Kitada, Masaaki ;
Kohama, Misaki ;
Matsue, Dai ;
Teramoto, Noboru ;
Ose, Takayuki ;
Itokazu, Yutaka ;
Koshino, Kazuhiro ;
Watabe, Hiroshi ;
Iida, Hidehiro ;
Takamoto, Tomoaki ;
Tabata, Yasuhiko ;
Dezawa, Mari .
EXPERIMENTAL NEUROLOGY, 2010, 223 (02) :537-547
[2]   Multipotent mesenchymal stromal cells attenuate chronic inflammation and injury-induced sensitivity to mechanical stimuli in experimental spinal cord injury [J].
Abrams, M. Birdsall ;
Dominguez, Cecilia ;
Pernold, Karin ;
Regera, Roxanne ;
Wiesenfeld-Hallin, Zsuzsanna ;
Olson, Lars ;
Prockop, Darwin .
RESTORATIVE NEUROLOGY AND NEUROSCIENCE, 2009, 27 (04) :307-321
[3]  
A comparison of autologous and allogenic bone marrow-derived mesenchymal stem cell transplantation in canine spinal cord injury.[J].Dong-In Jung;Jeongim Ha;Byeong-Teck Kang;Ju-Won Kim;Fu-Shi Quan;Jong-Hwan Lee;Eung-Je Woo;Hee-Myung Park.Journal of the Neurological Sciences.2009, 1
[4]  
Treatment of rat spinal cord injury with a Rho-kinase inhibitor and bone marrow stromal cell transplantation.[J].Takeo Furuya;Masayuki Hashimoto;Masao Koda;Akihiko Okawa;Atsushi Murata;Kazuhisa Takahashi;Toshihide Yamashita;Masashi Yamazaki.Brain Research.2009,
[5]  
Fate of transplanted adult neural stem/progenitor cells and bone marrow–derived mesenchymal stromal cells in the injured adult rat spinal cord and impact on functional recovery.[J].Ann M. Parr;Iris Kulbatski;Xing-Hua Wang;Armand Keating;Charles H. Tator.Surgical Neurology.2008, 6
[6]   Functional outcome of bone marrow stem cells (CD45+/CD34-) after cell therapy in acute spinal cord injury:: In exercise training and in sedentary rats [J].
Carvalho, K. A. T. ;
Cunha, R. C. ;
Vialle, E. N. ;
Osiecki, R. ;
Moreira, G. H. G. ;
Simeoni, R. B. ;
Francisco, J. C. ;
Guarita-Souza, L. C. ;
Oliveira, L. ;
Zocche, L. ;
Olandoski, M. .
TRANSPLANTATION PROCEEDINGS, 2008, 40 (03) :847-849
[7]   Differential effects of endogenous brain-derived neurotrophic factor on the survival of axotomized sensory neurons in dorsal root ganglia: A possible role for the P75 neurotrophin receptor [J].
Zhou, XF ;
Li, WP ;
Zhou, FHH ;
Zhong, JH ;
Mi, JX ;
Wu, LLY ;
Xian, CJ .
NEUROSCIENCE, 2005, 132 (03) :591-603
[8]   Marrow stromal cell transplantation after traumatic brain injury promotes cellular proliferation within the brain [J].
Mahmood, A ;
Lu, D ;
Chopp, M .
NEUROSURGERY, 2004, 55 (05) :1185-1192
[9]   Remyelination of the spinal cord following intravenous delivery of bone marrow cells [J].
Akiyama, Y ;
Radtke, C ;
Honmou, O ;
Kocsis, JD .
GLIA, 2002, 39 (03) :229-236
[10]   Transplantation of an acutely isolated bone marrow fraction repairs demyelinated adult rat spinal cord axons [J].
Sasaki, M ;
Honmou, O ;
Akiyama, Y ;
Uede, T ;
Hashi, K ;
Kocsis, JD .
GLIA, 2001, 35 (01) :26-34