用于莱茵衣藻荧光定量PCR分析的内参基因选择

被引：22

作者：

吴文凯 ^{[1
,2
]}

刘成前 ^{[2
]}

周志刚 ^{[1
]}

卢山 ^{[2
]}

机构：

[1] 上海海洋大学水产与生命学院

[2] 南京大学生命科学学院

来源：

植物生理学通讯 | 2009年 / 45卷 / 07期

关键词：

莱茵衣藻; 荧光定量PCR; 内参基因;

D O I：

暂无

中图分类号：

Q943 [植物细胞遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

本文用GeNorm与NormFinder算法分析actin、α-tubulin、cblp和yptc1四个常用内参基因在不同处理下莱茵衣藻中的表达。结果显示,α-tubulin在各种条件下都有较好的表达稳定性,而以不同基因组合构成的多内参系统可能更有利于细胞周期短暂的单细胞绿藻的分析。

引用

页码：667 / 672

页数：6

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