透明质酸改性壳聚糖-胶原-羟基磷灰石复合支架力学特性及成骨细胞的增殖(英文)

被引：11

作者：

刘璐 ^{[1
,2
]}

李瑞欣 ^{[1
]}

张莉 ^{[2
]}

郭勇 ^{[1
]}

陈学忠 ^{[1
]}

王亮 ^{[1
]}

董丽芝 ^{[1
]}

张西正 ^{[1
]}

机构：

[1] 解放军军事医学科学院卫生装备研究所

[2] 武警医学院

来源：

中国组织工程研究与临床康复 | 2011年 / 15卷 / 38期

关键词：

成骨细胞; 复合支架; 弹性模量; 细胞增殖; 骨组织工程;

D O I：

暂无

中图分类号：

R318.08 [生物材料学];

学科分类号：

100103 [病原生物学];

摘要：

背景:组织工程中,种子细胞需依赖于细胞外基质的存在才能发挥功能。因此支架材料的选择具有重大意义。目的:制备一种新型改性壳聚糖-胶原-羟基磷灰石复合支架,优化易于细胞黏附的组织工程支架材料工艺。方法:壳聚糖与透明质酸进行交联,红外和差示扫描量热图谱检测其结构;改性壳聚糖与胶原按1:2,1:1和2:1制备3种改性壳聚糖-胶原-羟基磷灰石复合支架,将复合支架与成骨细胞MC3T3-E1联合培养,CCK-8法检测增殖,绘制生长曲线。结果与结论:透明质酸和壳聚糖以酰胺键形成交联的新化合物,孔径在50～250μm之间,孔隙率随着胶原水平、弹性模量的增加而增加,而密度则减少;增加胶原的含量在细胞联合培养初期有利于细胞对支架的黏附和增殖,但从第10天开始,3种样品中细胞数量相差不大,均出现平台期;苏木精-伊红染色发现成骨细胞在培养初期沿着支架材料内部空隙贴壁生长,随着培养天数的增加,贴壁细胞呈集落样生长,可明显看到细胞间连接。说明透明质酸改性壳聚糖/胶原/纳米羟基磷灰石复合材料可以作为骨支架材料供成骨细胞黏附、增殖,其中胶原与壳聚糖的体积比为1:1为较优配比。

引用

页码：7127 / 7131

页数：5

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