PI3K/Akt参与内皮祖细胞分化的体外观察

被引：4

作者：

马颖

杨向红

机构：

[1] 中国医科大学附属盛京医院病理科

来源：

中国组织工程研究与临床康复 | 2009年 / 13卷 / 24期

关键词：

内皮祖细胞; 信号传导通路; PI3K/Akt; 分化;

D O I：

暂无

中图分类号：

R329.2 [人体细胞学];

学科分类号：

100101 ;

摘要：

背景:内皮祖细胞分化为成熟内皮细胞的分子机制尚不清楚,要通过包括PI3K/Akt信号通路在内的多个信号通路调节细胞内部多种基因的表达来完成。目的:分析PI3K/Akt信号传导通路在内皮祖细胞分化中的作用。设计、时间及地点:分组对照体外实验,于2007-09/2008-03在中国医科大学附属盛京医院病理实验室完成。材料:4～6周龄Wistar大鼠,雌雄不限。方法:采用密度梯度离心法从大鼠骨髓分离内皮祖细胞,经差速取二次贴壁细胞接种于培养瓶内。收集培养5d的内皮祖细胞,用激光共聚焦显微镜鉴定,AC133和vWF双染阳性细胞为正在分化的内皮祖细胞。主要观察指标:应用Western blot和反转录-聚合酶链反应检测培养0,3,7,10,14d的内皮祖细胞中AC133,vWF,PI3K,Akt蛋白和mRNA表达水平。结果:经Western blot和反转录-聚合酶链反应检测,AC133在培养0d表达最强,3d有弱表达,7,10,14d几乎没有表达(P<0.05);vWF在培养过程中表达强度没有明显变化(P>0.05);PI3K和Akt在培养0d表达最强,3d表达稍弱,随着培养时间的延长,表达逐渐减弱。PI3K/Akt与AC133表达趋势一致。结论:在内皮祖细胞分化为内皮细胞过程中,可能有信号传导通路PI3K/Akt的参与。

引用

页码：4704 / 4708

页数：5

共 9 条

[1] Common endothelial progenitor cell assays identify discrete endothelial progenitor cell populations [J].

Povsic, Thomas J. ;

Zavodni, Katherine L. ;

Vainorius, Enrikas ;

Kherani, Jennifer F. ;

Goldschmidt-Clermont, Pascal J. ;

Peterson, Eric D. .

AMERICAN HEART JOURNAL, 2009, 157 (02) :335-344

[2]

Pharmacological approaches to improve endothelial repair mechanisms[J] . Besler,Doerries,Giannotti,Lüscher,Landmesser.Expert Review of Cardiovascular Therapy . 2008 (8)

[3]

The Increase of Endothelial Progenitor Cells in the Peripheral Blood: A New Parameter for Detecting Onset and Severity of Sepsis[J] . C. Becchi.International Journal of Immunopathology and Phar . 2008 (3)

[4] Circulating endothelial progenitor cells correlate to stage in patients with invasive breast cancer [J].

Naik, Rakhi P. ;

Jin, David ;

Chuang, Ellen ;

Gold, Ellen G. ;

Tousimis, Eleni A. ;

Moore, Anne L. ;

Christos, Paul J. ;

de Dalmas, Tatiana ;

Donovan, Diana ;

Rafii, Shahin ;

Vahdat, Linda T. .

BREAST CANCER RESEARCH AND TREATMENT, 2008, 107 (01) :133-138

[5] Circulating endothelial progenitor cells in patients with Eisenmenger syndrome and idiopathic pulmonary arterial hypertension [J].

Diller, Gerhard-Paul ;

van Eijl, Sven ;

Okonko, Darlington O. ;

Howard, Luke S. ;

Ali, Omar ;

Thum, Thomas ;

Wort, Stephen J. ;

Bedard, Elisabeth ;

Gibbs, J. Simon R. ;

Bauersachs, Johann ;

Hobbs, Adrian J. ;

Wilkins, Martin R. ;

Gatzoulis, Michael A. ;

Wharton, John .

CIRCULATION, 2008, 117 (23) :3020-3030

[6] Endothelial progenitor cells in cardiovascular disorders [J].

Shantsila, Eduard ;

Watson, Timothy ;

Lip, Gregory Y. H. .

JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 2007, 49 (07) :741-752

[7]

Human CD34 + AC133 + VEGFR-2 + cells are not endothelial progenitor cells but distinct, primitive hematopoietic progenitors[J] . Jamie Case,Laura E. Mead,Waylan K. Bessler,Daniel Prater,Hilary A. White,M. Reza Saadatzadeh,Janak R. Bhavsar,Mervin C. Yoder,Laura S. Haneline,David A. Ingram.Experimental Hematology . 2007 (7)

[8] Bone marrow origin of endothelial progenitor cells responsible for postnatal vasculogenesis in physiological and pathological neovascularization [J].

Asahara, T ;

Masuda, H ;

Takahashi, T ;

Kalka, C ;

Pastore, C ;

Silver, M ;

Kearne, M ;

Magner, M ;

Isner, JM .

CIRCULATION RESEARCH, 1999, 85 (03) :221-228

[9]

Role of progenitor endothelial cells in cardiovasculardisease and upcoming therapies. Kawamoto A,Asahara T, et al. Catheterization and Cardiovascular Interventions . 2007

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