细胞质雄性不育高粱叶绿体ndh D基因的序列变异(英文)

被引：15

作者：

范昌发

孙春昀

郭骁才

张福耀

孙毅

牛天堂

贾敬芬

机构：

[1] 中国科学院成都生物研究所,中国科学院成都生物研究所,中国科学院成都生物研究所,山西省农业科学院高粱研究所,山西省农业科学院农业生物技术中心,山西省农业科学院农业生物技术中心,西北大学生命科学学院成都,成都,成都,榆次,太原,太原,西安

来源：

遗传学报 | 2002年 / 10期

关键词：

高粱; 细胞质雄性不育; 叶绿体ndhD、psaC基因; 序列变异;

D O I：

暂无

中图分类号：

Q943 [植物细胞遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

片段SAAU - 0 2 70 0 特异地扩增自 7种具可育细胞质的高粱材料的总DNA ,含有叶绿体psaC(88bp)和ndhD(192bp)基因的部分序列。该片段与EcoRⅠ +HindⅢ酶切的总DNA、线粒体DNA和叶绿体DNA杂交 ,在总DNA中获得了 0 .74kb的杂交带 ,而在叶绿体中获得 0 .74kb和 0 .4 5kb两条杂交带 ,与线粒体DNA无杂交 ;与经HaeⅢ酶切的总DNA杂交 ,在不育系中获得 4 .9kb的杂交带 ,而保持系的杂交带为 4 .4 5kb。参考GenBank中高粱的近缘物种玉米叶绿体基因组的序列 ,构建了ndhD基因区的酶切位点图谱 ,借此分析得出高粱不育系的叶绿体ndhD基因序列已发生改变。这种变异与高粱细胞质雄性不育发生的关系正在探讨中。

引用

页码：907 / 914

页数：8

共 10 条

[1] A型高粱细胞质雄性不育系与其保持系的胞质DNA和核DNA差异 [J].

范昌发 ;

孙春昀 ;

郭骁才 ;

张福耀 ;

牛天堂 .

应用与环境生物学报, 2001, (03) :291-296

[2] Brassica nap cytoplasmic male sterility is associated with expression of a mtDNA region containing a chimeric gene similar to the pol CMS-associated orf224 gene [J].

LHomme, Y ;

Stahl, RJ ;

Li, XQ ;

Hameed, A ;

Brown, GG .

CURRENT GENETICS, 1997, 31 (04) :325-335

[3] Cloning and sequencing of RAPD fragments amplified from mitochondrial DNA of male-sterile and male-fertile cytoplasm of sugar beet (Beta vulgaris L) [J].

Lorenz, M ;

Weihe, A ;

Borner, T .

THEORETICAL AND APPLIED GENETICS, 1997, 94 (02) :273-278

[4]

Sorghum mitochondrial orf25 and a related chimeric configuration of a male-sterile cytoplasm.[J].Hoang Tang;Daryl R. Pring;Figuhr R. Muza;Bin Yan.Current Genetics.1995, 3

[5]

Mapping of a chloroplast RFLP marker associated with the CMS cytoplasm of sugar beet ( Beta vulgaris ).[J].Z. Ran;G. Michaelis.Theoretical and Applied Genetics.1995, 6

[6]

A chloroplast DNA deletion located in RNA polymerase gene rpoC2 in CMS lines of sorghum.[J].Zengjian Chen;S. Muthukrishnan;George H. Liang;Keith F. Schertz;Gary E. Hart.MGG Molecular & General Genetics.1993, 2

[7] PARTIAL CONSERVATION OF THE 5' NDHE-PSAC-NDHD 3' GENE ARRANGEMENT OF CHLOROPLASTS IN THE CYANOBACTERIUM SYNECHOCYSTIS SP PCC-6803 - IMPLICATIONS FOR NDH-D FUNCTION IN CYANOBACTERIA AND CHLOROPLASTS [J].

ANDERSON, SL ;

MCINTOSH, L .

PLANT MOLECULAR BIOLOGY, 1991, 16 (04) :487-499

[8] A NADH DEHYDROGENASE SUBUNIT GENE IS CO-TRANSCRIBED WITH THE ABNORMAL PETUNIA MITOCHONDRIAL GENE ASSOCIATED WITH CYTOPLASMIC MALE-STERILITY [J].

RASMUSSEN, J ;

HANSON, MR .

MOLECULAR & GENERAL GENETICS, 1989, 215 (02) :332-336

[9] CORRECTION OF CHLOROPHYLL-DEFECTIVE MALE-STERILE WINTER OILSEED RAPE (BRASSICA-NAPUS) THROUGH ORGANELLE EXCHANGE - MOLECULAR ANALYSIS OF THE CYTOPLASM OF PARENTAL LINES AND CORRECTED PROGENY [J].

JARL, CI ;

VANGRINSVEN, MQJM ;

VANDENMARK, F .

THEORETICAL AND APPLIED GENETICS, 1989, 77 (01) :135-141

[10]

Comparison of the mitochondrial genome of Nicotiana tabacum with its progenitor species.[J].M. M. Bland;D. F. Matzinger;C. S. Levings.Theoretical and Applied Genetics.1985, 5

← 1 →