新的水稻谷蛋白α-1亚基缺失突变体(英文)

被引:12
作者
曲乐庆
魏晓丽
佐藤光
熊丸敏博
小川雅广
贾旭
机构
[1] 中国科学院遗传研究所植物细胞与染色体工程国家重点实验室!北京,中国科学院遗传研究所植物细胞与染色体工程国家重点实验室!北京,日本国立九州大学农学部!福冈-,日本国立九州大学农学部!福冈-,日本山口县立大学生命科学部!山口市-,中国科学
关键词
水稻谷蛋白突变体; SDS聚丙烯酰胺凝胶电泳; 等电聚焦电泳;
D O I
暂无
中图分类号
Q754 [基因的结构和突变的分子原理];
学科分类号
071007 ;
摘要
从水稻受精卵MNU处理后代中获得4个谷蛋白α-1亚基缺失突变品系。SDS-PAGE和IEF分析表明这些突变体在共同缺失1条pI6.82多肽的同时,或形成新的多肽,或其他多肽表现量增加,这些突变体是由结构基因控制的。IEF分析同时显示2条多肽PI6.82和PI8.58源自同一条谷蛋白前驱体。这4个突变体对于改良水稻谷蛋白品质、研究谷蛋白生物合成遗传调控机制以及揭示谷蛋白基因功能是不可多得的研究材料。
引用
收藏
页码:229 / 235
页数:7
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