双子表面活性剂及其保护的纳米金作为缓冲添加剂用于毛细管电泳蛋白质分离

被引：5

作者：

刘倩

李燕清

杨艳敏

姚守拙

机构：

[1] 湖南大学化学化工学院,化学生物传感与计量学国家重点实验室

来源：

中国科学(B辑:化学) | 2009年 / 39卷 / 10期

关键词：

双子表面活性剂; 蛋白质分离; 毛细管电泳; 金纳米粒子;

D O I：

暂无

中图分类号：

O658.9 [其他];

学科分类号：

070302 ; 081704 ;

摘要：

报道了使用阳离子双子表面活性剂作为毛细管电泳的缓冲添加剂用于同时分离酸性和碱性蛋白质.在酸性的缓冲条件下,只需要使用低浓度的阳离子双子表面活性剂(0.1mmol/L18-s-18)作为缓冲液的添加剂,就可以有效地抑制酸性和碱性蛋白质在毛细管壁的吸附,从而得到高效的蛋白质分离.实验表明,较小的胶束尺寸(如s=5～8)比大的胶束尺寸(如s<4或>10)能更有效地抑制酸性蛋白质的吸附.改变双子表面活性剂的中间基的长度能够对蛋白质的电泳淌度进行一定的调节,从而对分离的选择性进行一定的优化.在最优的实验条件下,蛋白质迁移时间的日内和日间标准偏差(RSD)分别小于0.8%和2.2%,回收率为79%到100.4%.另外,还考察了双子表面活性剂保护的金纳米颗粒用作毛细管电泳缓冲添加剂在蛋白质分离中的应用.实验表明,在缓冲液中加入纳米金能够缩短分析时间,并能小幅度地提高分离效率.最后,使用该方法分析了一系列复杂生物样品,包括血浆、红细胞和鸡蛋清样品,均得到了满意的结果.

引用

页码：1251 / 1261

页数：11

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