重复经颅磁刺激对慢性背根神经节受压致神经病理性疼痛大鼠的镇痛作用和机制

被引：7

作者：

张也 ^{[1
]}

赵丹 ^{[1
]}

许东升 ^{[1
,2
]}

机构：

[1] 同济大学附属同济医院康复中心

[2] 同济大学附属同济医院骨科

来源：

第二军医大学学报 | 2021年 / 42卷 / 07期

关键词：

重复经颅磁刺激; 神经病理性疼痛; 背根神经节; 脊髓背角; 星形胶质细胞; 突触后致密区95; 炎症因子;

D O I：

10.16781/j.0258-879x.2021.07.0749

中图分类号：

R402 [];

学科分类号：

摘要：

目的探讨重复经颅磁刺激（rTMS）对慢性背根神经节受压（CCD）致神经病理性疼痛（NPP）大鼠模型的镇痛作用及机制。方法将36只10周龄雄性SD大鼠随机分为3组:假手术+假刺激组、NPP+假刺激组、NPP+rTMS组,每组成功纳入12只。假手术+假刺激组和NPP+假刺激组大鼠上午、下午均接受假刺激,NPP+rTMS组大鼠上午接受假刺激,下午接受高频rTMS（大脑皮质M1区,20 Hz）。在CCD致NPP模型建立后第8天开始对大鼠进行rTMS真刺激或假刺激干预,连续11 d。rTMS干预前后用von Frey纤维丝和条件性位置偏爱实验分别测定机械痛阈和自发痛。在造模后第20天取大鼠左侧L4～L5节段背根神经节（DRG）及脊髓背角和血清标本,用蛋白质印迹法检测DRG和脊髓背角中卫星胶质细胞和星形胶质细胞活化标志物胶质纤维酸性蛋白（GFAP）及脊髓背角中突触后致密区95（PSD-95）蛋白的表达,ELISA检测血清中促炎因子IL-18和TNF-α水平。结果与假手术+假刺激组相比,NPP+假刺激组大鼠机械痛阈降低,DRG中GFAP表达增加,脊髓背角中GFAP和PSD-95蛋白表达均增加,血清中IL-18、TNF-α水平均增高,差异均有统计学意义（P均<0.05）;两组大鼠对A、B箱均无偏爱。而NPP+rTMS组（即高频rTMS干预后）大鼠上述改变与NPP+假刺激组相比均被逆转,且大鼠对B箱产生明显偏爱（P均<0.05）。结论高频rTMS可能通过抑制DRG中卫星胶质细胞活化、抑制脊髓背角中星形胶质细胞活化和突触连接形成及改善炎症微环境,缓解CCD导致的大鼠NPP。

引用

页码：749 / 754

页数：6

共 18 条

[1]

New procedure of high-frequency repetitive transcranial magnetic stimulation for central neuropathic pain: a placebo-controlled randomized crossover study [J].

Quesada, Charles ;

Pommier, Benjamin ;

Fauchon, Camille ;

Bradley, Claire ;

Creac'h, Christelle ;

Murat, Marion ;

Vassal, Francois ;

Peyron, Roland .

PAIN, 2020, 161 (04) :718-728

[2]

Analgesia-enhancing effects of repetitive transcranial magnetic stimulation on neuropathic pain after spinal cord injury: An fNIRS study [J].

Sun, Xiaolong ;

Long, Hua ;

Zhao, Chenguang ;

Duan, Qiang ;

Zhu, Huilin ;

Chen, Chunyan ;

Sun, Wei ;

Ju, Fen ;

Sun, Xinyan ;

Zhao, Yilin ;

Xue, Baijie ;

Tian, Fei ;

Mou, Xiang ;

Yuan, Hua .

RESTORATIVE NEUROLOGY AND NEUROSCIENCE, 2019, 37 (05) :497-507

[3]

Radicular Pain Syndromes: Cervical, Lumbar, and Spinal Stenosis [J].

Patel, Ekta A. ;

Perloff, Michael D. .

SEMINARS IN NEUROLOGY, 2018, 38 (06) :634-639

[4]

Interaction between astrocytic colony stimulating factor and its receptor on microglia mediates central sensitization and behavioral hypersensitivity in chronic post ischemic pain model.[J].Yuying Tang;Lian Liu;Dan Xu;Wensheng Zhang;Yi Zhang;Jieshu Zhou;Wei Huang.Brain Behavior and Immunity.2018,

[5]

IKK/NF-κB-dependent satellite glia activation induces spinal cord microglia activation and neuropathic pain after nerve injury [J].

Lim, Hyoungsub ;

Lee, Hyunkyoung ;

Noh, Kyungchul ;

Lee, Sung Joong .

PAIN, 2017, 158 (09) :1666-1677

[6]

Perturbing NR2B-PSD-95 interaction relieves neuropathic pain by inactivating CaMKII-CREB signaling [J].

Xu, Fangxia ;

Zhao, Xin ;

Liu, Lin ;

Song, Jia ;

Zhu, Yingjun ;

Chu, Shuaishuai ;

Shao, Xueming ;

Li, Xiuxiu ;

Ma, Zhengliang ;

Gu, Xiaoping .

NEUROREPORT, 2017, 28 (13) :856-863

[7]

Low level laser therapy alters satellite glial cell expression and reverses nociceptive behavior in rats with neuropathic pain [J].

Oliveira, M. E. ;

Santos, F. M. ;

Bonifacio, R. P. ;

Freitas, M. F. ;

Martins, D. O. ;

Chacur, M. .

PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, 2017, 16 (04) :547-554

[8]

Effects of the dimeric PSD-95 inhibitor UCCB01-144 in mouse models of pain; cognition and motor function.[J].Jesper T. Andreasen;Arafat Nasser;Maitane Caballero-Puntiverio;Maj Sahlholt;Anders Bach;Mikko Gynther;Kristian Strømgaard;Darryl S. Pickering.European Journal of Pharmacology.2016,

[9]

Pain and the global burden of disease [J].