Self-Assembled Multilayers of Polyethylenimine and DNA: Spectrophotometric and Electrochemical Characterization and Application for the Determination of Acridine Orange Interaction

被引：6

作者：

Ferreyra, N. F. ^{[1
]}

Rivas, G. A. ^{[1
]}

机构：

[1] Univ Nacl Cordoba, Fac Ciencias Quim, INFIQC, Dept Fisicoquim, RA-5000 Cordoba, Argentina

来源：

ELECTROANALYSIS | 2009年 / 21卷 / 15期

关键词：

DNA; Polyethylenimine; Self-assembled multilayers; Acridine orange; Intercalation; Gold; DNA interaction; BIOSENSORS; VOLTAMMETRY; CARBON; FILMS; DERIVATIVES; ELECTRODES; BINDING; MERCURY;

D O I：

10.1002/elan.200904593

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This work deals with the study of the interaction between acridine orange (AO) and calf-thymus double stranded DNA (dsDNA) present in supramolecular architectures built on gold electrodes modified with mercapto-1-propanesulfonic acid (MPS) by self-assembling of polyethylenimine and dsDNA. The optimal conditions for building the supramolecular architecture were obtained from UV-vis spectrophotometric experiments. The electrochemical studies were performed by adsorptive transfer square wave voltammetry from the evaluation of the oxidation signal of AO accumulated within the multistructure. The effect of the number of PEI-dsDNA bilayers (Au/MPS/(PEI-dsDNA)(n)) on the accumulation and electrooxidation of AO is also discussed.

引用

页码：1665 / 1671

页数：7

共 38 条

[1] Electrochemical biosensors for medical and food applications
Ahmed, Minhaz Uddin
Hossain, Mohammad Mosharraf
Tamiya, Eiichi
[J]. ELECTROANALYSIS, 2008, 20 (06) : 616 - 626
[2] Layer-by-layer assembly as a versatile bottom-up nanofabrication technique for exploratory research and realistic application
Ariga, Katsuhiko
Hill, Jonathan P.
Ji, Qingmin
[J]. PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2007, 9 (19) : 2319 - 2340
[3] Electrochemical biosensors based on nucleic acids and their use in bioaffinity assays for determining DNA and its effectors
Babkina, S. S.
Budnikov, G. K.
[J]. JOURNAL OF ANALYTICAL CHEMISTRY, 2006, 61 (08) : 728 - 739
[4] Acridine and acridone derivatives, anticancer properties and synthetic methods: Where are we now?
Belmont, Philippe
Bosson, Johann
Godet, Thomas
Tiano, Martin
[J]. ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY, 2007, 7 (02) : 139 - 169
[5] Electrooxidation of DNA at glassy carbon electrodes modified with multiwall carbon nanotubes dispersed in chitosan
Bollo, Soledad
Ferreyra, Nancy F.
Rivas, Gustavo A.
[J]. ELECTROANALYSIS, 2007, 19 (7-8) : 833 - 840
[6] Cordes REP, 2000, ELECTROANAL, V12, P351, DOI 10.1002/(SICI)1521-4109(20000301)12:5<351::AID-ELAN351>3.0.CO
[7] 2-I
[8] ACRIDINE-ORANGE ASSOCIATION EQUILIBRIUM IN AQUEOUS-SOLUTION
COSTANTINO, L
GUARINO, G
ORTONA, O
VITAGLIANO, V
[J]. JOURNAL OF CHEMICAL AND ENGINEERING DATA, 1984, 29 (01) : 62 - 66
[9] INTERACTIONS OF ACRIDINE ANTITUMOR AGENTS WITH DNA - BINDING-ENERGIES AND GROOVE PREFERENCES
CRENSHAW, JM
GRAVES, DE
DENNY, WA
[J]. BIOCHEMISTRY, 1995, 34 (41) : 13682 - 13687
[10] DECHER G, 2003, MULTILAYERS THIN FIL, pCH1

← 1 2 3 4 →