香兰素下调cGAS/STING信号通路改善肝内胆汁淤积大鼠肝组织损伤

被引：2

作者：

姜宁 ^{[1
]}

蒲兰香 ^{[2
]}

黄峰 ^{[3
]}

王艳 ^{[1
]}

裴鑫 ^{[1
]}

宋均亚 ^{[1
]}

张恩胜 ^{[1
]}

机构：

[1] 青岛大学附属山东省妇幼保健院儿科

[2] 青岛大学附属山东省妇幼保健院药剂科

[3] 武警山东省总队医院特色专业一科

来源：

中国药理学通报 | 2024年 / 40卷 / 09期

关键词：

香兰素; 环鸟苷酸腺苷酸合成酶; 干扰素基因刺激因子; 肝内胆汁淤积; 肝损伤; 肝纤维化;

D O I：

暂无

中图分类号：

R285.5 [中药实验药理];

学科分类号：

100806 [中药药理学];

摘要：

目的探讨香兰素调节环鸟苷酸腺苷酸合成酶(cyclic guanylate adenylate synthetase, cGAS)/干扰素基因刺激因子(stimulator of interferon gene, STING)信号通路对肝内胆汁淤积(intrahepatic cholestasis, IC)大鼠肝组织损伤的影响。方法将SD大鼠随机分为正常组、IC组、香兰素组、cGAS过表达组、香兰素+cGAS过表达组，连续给药7 d。测定大鼠体质量、肝质量和肝质量/体质量比值；酶联免疫吸附法测定肝功能指标(ALT、AST、ALP、LDH),IC指标(TBIL、TBA),肝纤维化指标(HA、LN、PCⅢ);HE、Masson染色分别观察肝脏病理学、纤维沉积变化，并计算胶原容积分数；蛋白印迹法检测肝组织cGAS/STING通路相关蛋白表达。结果香兰素可改善IC大鼠肝脏病理、纤维化，升高体质量，降低肝脏质量、ALT、AST、ALP、LDH、TBIL、TBA、HA、LN、PCⅢ、胶原容积分数、cGAS、STING蛋白(P<0.05);cGAS过表达可逆转香兰素对IC大鼠上述指标的影响(P<0.05)。结论香兰素可能通过下调cGAS/STING信号通路，改善IC大鼠肝功能、IC、肝纤维化和肝组织损伤。

引用

页码：1695 / 1700

页数：6

共 18 条

[1]

电针干预经cGAS/STING通路对帕金森综合征大鼠神经功能的影响 [J].

刘艳阳 ;

刘佩军 ;

翟宏宇 ;

王珊珊 ;

郭雅碧 ;

王红 .

中国老年学杂志, 2023, 43 (08) :1909-1913

[2]

柴胡疏肝散调控FXR/Nrf2/ARE通路对肝内胆汁淤积大鼠肝损伤的保护作用研究.[J].娄静;赵雷;朱岩洁;袁帅强;王菲;张杭洲;徐娇娇;余晓珂;侯留法;.中国中药杂志.2022, 20

[3]

FOXA2改善肝内胆汁淤积伴高脂血症小鼠肝脏功能和血脂水平 [J].

张梦洁 ;

杨洋 ;

陈慧 ;

陈思聪 ;

翟华立 ;

杨晓明 ;

于淼 ;

焦轶 .

中国药理学通报, 2021, 37 (11) :1593-1599

[4]

Clinicopathologic Features, Genetics, Treatment, and Long-Term Outcomes in Japanese Children and Young Adults with Benign Recurrent Intrahepatic Cholestasis: A Multicenter Study [J].

Kato, Ken ;

Umetsu, Shuichiro ;

Togawa, Takao ;

Ito, Koichi ;

Kawabata, Takayoshi ;

Arinaga-Hino, Teruko ;

Tsumura, Naoya ;

Yasuda, Ryosuke ;

Mihara, Yutaro ;

Kusano, Hironori ;

Ito, Shogo ;

Imagawa, Kazuo ;

Hayashi, Hisamitsu ;

Inui, Ayano ;

Yamashita, Yushiro ;

Mizuochi, Tatsuki .

JOURNAL OF CLINICAL MEDICINE, 2023, 12 (18)

[5]

Naringenin is a Potential Immunomodulator for Inhibiting Liver Fibrosis by Inhibiting the cGAS-STING Pathway [J].

Chen, Li ;

Xia, Siwei ;

Wang, Shuqi ;

Zhou, Yuanyuan ;

Wang, Feixia ;

Li, Zhanghao ;

Li, Yang ;

Kong, Desong ;

Zhang, Zili ;

Shao, Jiangjuan ;

Xu, Xuefen ;

Zhang, Feng ;

Zheng, Shizhong .

JOURNAL OF CLINICAL AND TRANSLATIONAL HEPATOLOGY, 2023, 11 (01) :26-37

[6]

Intrahepatic cholestasis in sickle cell disease: A review of diagnostic criteria, treatments, and case reports [J].

Edwards, Christopher L. ;

Scott, Sharena ;

Boggan, Michaela ;

Meek, Jordan ;

Alston, Kiera ;

Pearson, Aiden ;

Mcdougald, Alexandria ;

Broadnax, Mckenzie ;

Wood, Mary ;

Barker, Camela S. ;

Miller, Jessica ;

Whitworth, Elaine ;

James, Osaffo ;

Sollers III, John J. ;

Bryson, W. Jeff ;

Thorpe, Roland ;

Byrd, Goldie ;

Whitfield, Keith E. ;

Sudhakar, Shiv ;

Parker, Debra O. ;

Livingston, Jonathan ;

Shah, Nirmish ;

Railey, Kenyon .

JOURNAL OF THE NATIONAL MEDICAL ASSOCIATION, 2023, 115 (01) :26-37

[7]

Ginsenoside Rd Inhibited Ferroptosis to Alleviate CCl4-Induced Acute Liver Injury in Mice via cGAS/STING Pathway [J].

Li, Yuangeng ;

Yu, Ping ;

Fu, Wenwen ;

Wang, Shuo ;

Zhao, Wenjun ;

Ma, Yue ;

Wu, Yi ;

Cui, Heming ;

Yu, Xiaofeng ;

Fu, Li ;

Xu, Huali ;

Sui, Dayun .

AMERICAN JOURNAL OF CHINESE MEDICINE, 2023, 51 (01) :91-105

[8]

Blocking cGAS/STING signaling protects against sepsis-associated acute liver injury [J].

Li, Jie ;

Lu, Yanyan ;

Lin, Ge .

INTERNATIONAL IMMUNOPHARMACOLOGY, 2022, 113

[9]

Diagnostic criteria for intrahepatic cholestasis of pregnancy: should the timing of total bile acids measurement be established?.[J].Huri Mor;Lippi Camilla;Seravalli Viola;Di Tommaso Mariarosaria.International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics.2022, 1

[10]

Replication stress triggered by nucleotide pool imbalance drives DNA damage and cGAS-STING pathway activation in NAFLD [J].

Donne, Romain ;

Saroul-Ainama, Maeva ;

Cordier, Pierre ;

Hammoutene, Adel ;

Kabore, Christelle ;

Stadler, Mira ;

Nemazanyy, Ivan ;

Galy-Fauroux, Isabelle ;

Herrag, Mounia ;

Riedl, Tobias ;

Chansel-Da Cruz, Marie ;

Caruso, Stefano ;

Bonnafous, Stephanie ;

Ollinger, Rupert ;

Rad, Roland ;

Unger, Kristian ;

Tran, Albert ;

Couty, Jean-Pierre ;

Gual, Philippe ;

Paradis, Valerie ;

Celton-Morizur, Severine ;

Heikenwalder, Mathias ;

Revy, Patrick ;

Desdouets, Chantal .

DEVELOPMENTAL CELL, 2022, 57 (14) :1728-+

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