Discovery of a bioactive peptide, an angiotensin converting enzyme inhibitor in Chinese Baijiu

被引：19

作者：

Wu J. ^{[1
,2
,3
]}

Sun B. ^{[1
,2
]}

Zhao M. ^{[3
]}

Zheng F. ^{[1
,2
]}

Sun J. ^{[1
,2
]}

Sun X. ^{[1
,2
]}

Li H. ^{[1
,2
]}

Huang M. ^{[1
,2
]}

机构：

[1] Beijing Key Laboratory of Flavor Chemistry, Bejing Technology and Business University, Beijing

[2] Beijing Innovation Centre of Food Nutrition and Human Health, Beijing

[3] School of Food Science and Engineering, South China University of Technology, Guangzhou

来源：

Journal of Chinese Institute of Food Science and Technology | 2016年 / 16卷 / 09期

关键词：

ACE inhibitory; Bioactive peptide; Chinese Baijiu; Non-competitive; PHP;

D O I：

10.16429/j.1009-7848.2016.09.002

中图分类号：

学科分类号：

摘要：

The Chinese baijiu samples were concentrated with rotary evaporator, and a peptide with novel ACE inhibitory activity was discovered in the concentrated solutions for the first time. The sequence of amino acids was identified by liquid chromatography-ESI-mass spectrometry/mass spectrometry (LC-ESI-QTOF-MS) and synthetic standards. The ACE inhibitory activity assay was determined for the peptide by using the modified HPLC method. The inhibition pattern on ACE and IC50 of the bioactive peptide were also studied in the paper. The results showed that the novel ACE inhibitory peptide, which was composed of 3 amino acids, was discovered for the first time in Chinese Baijiu and the sequence of the peptide was Pro-His-Pro (PHP) with an IC50 value of 446 mmol/L. In addition, Lineweaver-Burk plots suggested that PHP played the role as a non-competitive inhibitor against ACE. © 2016, Editorial Office of Journal of CIFST. All right reserved.

引用

页码：14 / 20

页数：6

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