A quadrupole ion trap mass spectrometer with three independent ion sources for the study of gas-phase ion/ion reactions

被引：39

作者：

Badman, Ethan R. ^{[1
]}

Chrisman, Paul A. ^{[1
]}

McLuckey, Scott A. ^{[1
]}

机构：

[1] Department of Chemistry, Purdue University, West Lafayette, IN 47907-1393, United States

来源：

Analytical Chemistry | 2002年 / 74卷 / 24期

关键词：

Ionization - Ions - Manipulators - Mass spectrometers - Proteins;

D O I：

10.1021/ac026020w

中图分类号：

学科分类号：

摘要：

An instrument for the study of gas-phase ion/ion reactions in which three independent sources of ions, namely, two electrospray ionization sources and one atmospheric sampling glow discharge ionization source, are interfaced to a quadrupole ion trap mass analyzer is described. This instrument expands the scope of gas-phase ion/ion reaction studies by allowing for manipulation of the charge states of multiply charged reactant and product ions. Examples are provided involving the formation of protein protein complexes in the gas phase. Complexes with charge states that cannot be formed from reactant ion charge states present in the normal electrospray charge state distributions can be formed in the new apparatus. Strategies that rely on both reactant ion charge state manipulation and product ion charge state manipulation are demonstrated. In addition, simplification of product ion spectra generated from dissociation of complexes formed via ion/ion reactions can be effected by using the discharge source to reduce the charge state of the product ions to primarily 1+.

引用

页码：6237 / 6243