Modified cetyltrimethylammonium bromide method improves robustness and versatility: The benchmark for plant RNA extraction

被引：87

作者：

White, Elizabeth J. ^{[1
]}

Venter, Mauritz ^{[1
]}

Hiten, Nicholas F. ^{[2
]}

Burger, Johan T. ^{[1
]}

机构：

[1] Department of Genetics, Stellenbosch University, Matieland 7602

[2] Institute for Plant Biotechnology, Department of Genetics, Stellenbosch University, Matieland 7602

来源：

Biotechnology Journal | 2008年 / 3卷 / 11期

关键词：

Cetyltrimethylammonium bromide; Plant leaf; RNA; RT-PCR;

D O I：

10.1002/biot.200800207

中图分类号：

学科分类号：

摘要：

A wide range of plant RNA extraction methods are available; however, many of these are limited in their application for a diverse range of plant species. With special emphasis on robustness and versatility, we have improved the cetyltrimethylammonium bromide (CTAB) method and isolated high-quality RNA from 16 different plant species. The major modifications made to the protocol described here were a reduction of sample treatment steps and an increase in β-mercaptoethanol concentration (to 3%) resulting in a robust, rapid and reproducible plant RNA extraction protocol that can be used for a broad range of plant species and tissue types. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：1424 / 1428

页数：4

共 12 条

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