Spray-dried chitosan microspheres as a pDNA carrier

被引：34

作者：

State University of Campinas, School of Chemical Engineering, Campinas, SP, Brazil ^{[1
]}

不详 ^{[2
]}

机构：

[1] State University of Campinas, School of Chemical Engineering, Campinas, SP

[2] IPT- Institute for Technological Research of the State of Saõ Paulo, Laboratory Processes Chemical and Particle Technology, 05508-901 Saõ Paulo, SP

来源：

Dry. Technol. | 2006年 / 3卷 / 373-382期

基金：

巴西圣保罗研究基金会;

关键词：

Chitosan microspheres; Crosslinking; Glyceraldehyde; pDNA; Spray drying;

D O I：

10.1080/07373930600564480

中图分类号：

学科分类号：

摘要：

Spray-dried chitosan microparticles with controlled size were prepared and crosslinked with the biocompatible reactant d,l-glyceraldehyde. Glutaraldehyde, the conventional cross-linking agent for proteins, was used as a control. The crosslinking degree was characterized through thermal analysis, X-ray diffraction, and infrared spectroscopy. In addition, the crosslinking effects were analyzed in terms of particle size, swelling capability, and surface charge. By properly choosing d,l -glyceraldehyde concentration and crosslink time, a high degree of control was achieved over the physical properties of particles. Yields around 78% as well as images obtained through fluorescence microscopy demonstrated the capability of the crosslinked chitosan microspheres to pDNA complexation. Copyright © 2006 Taylor & Francis Group, LLC.

引用

页码：373 / 382

页数：9

共 25 条

[1]

He P., Davis S.S., Illum L., Chitosan microspheres prepared by spray-drying, International Journal of Pharmaceutics, 187, 1, pp. 53-65, (1999)

[2]

Van der Lubben I.M., Verhoef J.C., Borchard G., Junginger H.E., Chitosan and its derivatives in mucosal drug and vaccine delivery, European Journal of Pharmaceutical Sciences, 14, 3, pp. 201-207, (2001)

[3]

Huang Y.C., Chiang C.H., Yeh M.K., Optimizing formulation factors in preparing Chitosan microparticles by spray-drying method, Journal of Microencapsulation, 20, 2, pp. 247-260, (2003)

[4]

Adamiec J., Mdrzejewska Z., Some structural properties of spray-dried microgranules, Drying Technology, 23, pp. 1601-1611, (2005)

[5]

Huntington D.H., The influence of the spray drying process on product properties, Drying Technology, 22, pp. 1261-1287, (2004)

[6]

Masters K., Current market-driven spray drying development activities, Drying Technology, 22, pp. 1351-1370, (2004)

[7]

Chen X.D., Heat-mass transfer and structure formation during drying of single food droplets, Drying Technology, 22, pp. 179-190, (2004)

[8]

Re M.I., Microencapsulation by spray drying, Drying Technology, 16, 6, pp. 1195-1236, (1998)

[9]

Kim C.J., Lee P.I., Synthesis and characterization of suspension-polymerized Poly(Vinyl Alcohol) beads with core-shell structure, Journal of Applied Polymer Science, 46, 12, pp. 2147-2157, (1992)

[10]

Vandelli M.A., Pifferi G., Seghizzi R., Cameroni R., Swelling behaviour of gelatin microspheres crosslinked with d,l-glyceraldehyde. The effect of the preparative factors, Pharmacy and Pharmacology Letters, 5, pp. 116-119, (1995)

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