A storable encapsulated bilayer chip containing a single protein nanopore

被引：120

作者：

Department of Molecular and Cellular Medicine, Texas A and M University, System Health Science Center, College Station, TX 77843-1114, United States ^{[1
]}

不详 ^{[2
]}

不详 ^{[3
]}

机构：

[1] Department of Molecular and Cellular Medicine, Texas A and M University, System Health Science Center, College Station

[2] Department of Chemistry, University of Oxford

[3] Department of Chemistry, Michigan State University, East Lansing

来源：

J. Am. Chem. Soc. | 2007年 / 15卷 / 4701-4705期

基金：

英国医学研究理事会;

关键词：

Proteins;

D O I：

10.1021/ja068654g

中图分类号：

学科分类号：

摘要：

A robust, portable chip containing a single protein nanopore would be a significant development in the practical application of stochastic sensing technology. Here, we describe a chip in which a single α-hemolysin (αHL) pore in a planar phospholipid bilayer is sandwiched between two layers of agarose gel. These encapsulated nanopore chips remain functional after storage for weeks. The detection of the second messenger inositol 1,4,5-trisphosphate (IP3) was demonstrated with a chip containing a genetically engineered αHL pore as the sensor element. © 2007 American Chemical Society.

引用

页码：4701 / 4705

页数：4

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