Multiplex pathogen detection based on spatially addressable microarrays of barcoded resins

被引：13

作者：

Steacie Institute for Molecular Sciences, National Research Council of Canada, 100 Sussex Drive, Ottawa, ON K1A 0R6, Canada ^{[1
]}

不详 ^{[2
]}

机构：

[1] Steacie Institute for Molecular Sciences, National Research Council of Canada, Ottawa, ON K1A 0R6

[2] National Institute for Nanotechnology, National Research Council, University of Alberta, Edmonton, AB

来源：

Biotechnol. J. | 2008年 / 7卷 / 948-953期

关键词：

Antigens; Barcoded resins; Biothreat pathogens; Multiplex immunoassays; Raman spectroscopy;

D O I：

10.1002/biot.200700236

中图分类号：

学科分类号：

摘要：

Suspension microsphere immunoassays are rapidly gaining recognition in antigen identification and infectious disease biodetection due to their simplicity, versatility and high-throughput multiplex screening. We demonstrate a multiplex assay based on antibody-functionalized barcoded resins (BCRs) to identify pathogen antigens in complex biological fluids. The binding event of a particular antibody on given bead (fluorescence) and the identification of the specific pathogen agent (vibrational fingerprint of the bead) can be achieved in a dispersive Raman system by exciting the sample with two different laser lines. Anthrax protective antigen, Franciscella tularensis lipopolysaccharide and CD14 antigens were accurately identified and quantified in tetraplex assays with a detection limit of 1 ng/mL. The rapid, versatile and simple analysis enabled by the BCRs demonstrates their potential for multiplex antigen detection and identification in a reconfigurable microarray format. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：948 / 953

页数：5

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