Development of a novel biosensor for the detection of arsenic in drinking water

被引：20

作者：

School of Biological Sciences, University of Edinburgh, Edinburgh, United Kingdom ^{[1
]}

不详 ^{[2
]}

不详 ^{[3
]}

机构：

[1] School of Biological Sciences, University of Edinburgh, Edinburgh

[2] School of Informatics, University of Edinburgh, Edinburgh

[3] School of Engineering and Electronics, University of Edinburgh, Edinburgh

来源：

IET Synth. Biol. | 2007年 / 1-2卷 / 87-90期

关键词：

13;

D O I：

10.1049/iet-stb:20060002

中图分类号：

学科分类号：

摘要：

We sought to develop a whole-cell biosensor for the detection of arsenic in drinking water, a major problem in Bangladesh and West Bengal. In contrast to previously described systems, our biosensor would give a pH change as output, allowing simple detection with a pH electrode or pH indicator solution. We designed and modelled a system based on the arsenate-responsive promoter of the Escherichia coli arsenic detoxification system, using urease to increase pH in the absence of arsenate, and β-galactosidase (LacZ) to decrease pH in the presence of arsenate. The pH-reducing β-galactosidase part of the system was constructed and tested, and was found to give a clear response to arsenate concentrations as low as 5ppb arsenic, well below the World Health Organisation (WHO) recommended limit of 10ppb. © 2007 The Institution of Engineering and Technology.

引用

页码：87 / 90

页数：3

共 13 条

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