Wide-field optically sectioning fluorescence microscopy with laser illumination

被引：67

作者：

Neil, M.A.A. ^{[1
]}

Squire, A. ^{[1
,2
]}

Juškaitis, R. ^{[1
]}

Bastiaens, P.I.H. ^{[1
,2
]}

Wilson, T. ^{[1
]}

机构：

[1] Department of Engineering Science, University of Oxford, Parks Road, Oxford OX1 3PJ, United Kingdom

[2] Cell Biophysics Laboratory, Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, London WC2A 3PX, United Kingdom

来源：

Journal of Microscopy | 2000年 / 197卷 / 01期

关键词：

Fluorescence;

D O I：

10.1046/j.1365-2818.2000.00656.x

中图分类号：

学科分类号：

摘要：

We describe an extremely simple method by which optically sectioned fluorescence images may be obtained with conventional microscopes using laser illumination. A one-dimensional grid pattern is introduced into the illumination system, together with a rotating ground glass diffuser. This causes an image of the grid pattern to be projected into the specimen. Images taken at three spatial positions of the grid are processed in a simple manner to provide optically sectioned images of fluorescent specimens.

引用

页码：1 / 4