Dextran modified gold surfaces for surface plasmon resonance sensors. Immunoreactivity of immobilized antibodies and antibody. Surface interaction studies

被引：46

作者：

Loefaes, Stefan ^{[1
]}

Johnsson, Bo ^{[1
]}

Tegendal, Katarina ^{[1
]}

Roennberg, Inger ^{[1
]}

机构：

[1] Pharmacia Biosensor, Uppsala, Sweden

来源：

Colloids and Surfaces B: Biointerfaces | 1993年 / 1卷 / 02期

关键词：

Antibodies - Chemical modification - Gold - Immunology - Polysaccharides - Surface properties - Surface treatment;

D O I：

10.1016/0927-7765(93)80038-Z

中图分类号：

学科分类号：

摘要：

Recently, the modification of gold surfaces with covalently bound carboxylated dextran was described, for use in biospecific interaction studies in an analytical system based on surface plasmon resonance (SPR). In this study, the antigen binding immunoreactivity of antibodies immobilized on the modified surfaces was investigated with the SPR-based system. Four different monoclonal antibodies specific against the antigens β2-microglobulin, luteinizing hormone, human transferrin, and immunoglobulin E, were covalently attached by amine coupling to the dextran layer and the antigen binding capacities were evaluated for different amounts of bound antibody. High immunoreactivities were obtained with molar activities ranging from 0.6 to 1.5 over a wide range of surface concentrations. Interaction studies between carboxymethylated dextran surfaces and antibodies showed that, in comparison with unmodified gold surfaces, the modified surface had low nonspecific adsorption and high capacity for antibody immobilization.

引用

页码：83 / 89